Banca de DEFESA: MICHELE CAGNIN VICENTE

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : MICHELE CAGNIN VICENTE
DATA : 26/06/2019
HORA: 14:00
LOCAL: Sala de defesa do PPGF
TÍTULO:

In vitro propagation and acclimatization of Cattleya walkeriana Gardner: species in the red list of Brazilian flora.

PALAVRAS-CHAVES:

orchids, preservation, culture media; microbiolization

PÁGINAS: 132
GRANDE ÁREA: Ciências Agrárias
ÁREA: Agronomia
SUBÁREA: Fitotecnia
ESPECIALIDADE: Produção de Mudas
RESUMO:

Cattleya walkeriana is an orchid endemic to Brazil of high commercial value and much coveted by orchids, facts that linked to the reproductive specificities of the family places it among the species of flora at risk of extinction high in nature. In this way the in vitro propagation is shown as a valuable tool to obtain seedlings, aiming at supplying the market and preserving the species. In this context we sought to evaluate the development of Cattleya walkeriana Gardner seedlings grown in different culture media in vitro propagation system and later the ex vitro performance by acclimatization in greenhouse. Seedlings approximately 1.0 cm in length were subcultured for 240 days in the Murashige & Skoog culture media, 1962 (MS); Knudson C, 1946 modified by Morel, 1965 (KC); Peters® Fertilizer (PE); Kristalon ™ Orange Fertilizer (KL); Culture medium B & G Orchidées® (BG). At the end of this period, the variables total fresh mass (MFT), number of leaves (NF), length of largest leaf (CMF), root number (NR), length of largest root (CMR) and final pH of culture (final pH). After these evaluations seedlings from the treatments MS, PE, KL and BG were taken to acclimatization in greenhouse where they were inoculated with 1.0 ml of the suspension of Azospirillum brasilense SP 245 BR 11005 (AZ); with 1.0 ml of the Microvirga vignae BR 3299 (MV) suspension; and with 1.0 ml of distilled H 2 O, the latter was designated Control (CO) treatment. The total fresh mass (MFT), number of leaves (NF), length of largest leaf (CMF), root number (total number of leaves) were evaluated. NR) and the length of the largest root (CMR). It was concluded that the Knudson C 1946 medium modified by Morel, 1965 is not suitable for in vitro propagation of Cattleya walkeriana seedlings under the conditions of this experiment. The B & G Orchidées® supply medium and the Kristalon ™ Orange fertilizer culture medium proved to be the most efficient in seedling development under in vitro conditions, but the Peters® fertilizer culture medium also proved to be a good alternative in substitution of the Murashige & Skoog, 1962 culture medium. However, when acclimatized the seedlings from the B & G Orchidées® supply medium were the ones that presented the best results regardless of the inoculation factor, therefore, it can be concluded that this medium was shown the most indicated in the in vitro propagation and the later acclimatization of the seedlings of Cattleya walkeriana.

MEMBROS DA BANCA:
Presidente - 2186690 - JOAO SEBASTIAO DE PAULA ARAUJO
Externo ao Programa - 2379124 - ROGERIO GOMES PEGO
Externo à Instituição - SANDY SAMPAIO VIDEIRA - UFRRJ