Micropropagation of Threatened Species of the Araceae Family: Strategy For Conservation of Live Collections
Fabric culture; living collections; extinction risk; Anthurium sp., Philodendrum sp.Living collections are an important form of conservation of endangered species. Among these, the Sítio Roberto Burle Marx stands out, which houses many native specimens, and some of them At Risk of Extinction (EN), with the Araceae family collection being one of the most important in this collection. It is known that the propagation of species of the Araceae family is slow and difficult to be carried out by traditional means, that is why tissue culture has been presented as the main alternative for multiplying these plants. Therefore, the objective of this work is to establish efficient micropropagation protocols for endangered species of the Araceae family and the production of plants for conservation and maintenance of living collections. For induction of calluses and sprouts, the MS culture medium with half the salt concentration of the original composition will be used, supplemented with 0.5, 1.0, 1.5 and 2.0 mg L-1 of 6-benzylaminopurine (BAP ) and 0.0, 0.1 mg L-1 of dichlorophenoxyacetic acid (2,4 - D), in all possible combinations. Plants obtained in this stage of multiplication will be subcultured to growth medium composed of MS medium supplemented by 0.0, 0.5, 1.0 1.5 and 2.0 mg L-1 of Indobutyric acid (IBA). And after 60 days of cultivation, they will be acclimatized on different substrates composed of sphagnum moss, coconut fiber, vermiculite or washed sand. Subsequently, different substrates will be tested for the cultivation of plants from micropropagation for subsequent return to living collections and redistribution in other protected areas in Brazil. With this research, it is expected to establish efficient protocols for the mass multiplication of endangered species of the Araceae family, contributing to the increase of these specimens in living collections as a conservation strategy.