Banca de DEFESA: GUSTAVO FEITOSA DE MATOS

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : GUSTAVO FEITOSA DE MATOS
DATE: 29/03/2021
TIME: 09:00
LOCAL: plataforma virtual Google Meet
TITLE:

Ecological and Functional Study of the Bradyrhizobium Strain Association with Sugarcane

KEY WORDS:

sugarcane, biological nitrogen fixation,  diazotrophic bacteria, crop rotation

PAGES: 150
BIG AREA: Ciências Agrárias
AREA: Agronomia
SUBÁREA: Fitotecnia
SPECIALTY: Fisiologia de Plantas Cultivadas
SUMMARY:

Bradyrhizobium is a genus of bacteria well known for its symbiotic association with cultures of the Fabaceae family (legumes), in which it demonstrates an efficient ability to fix itrogen from the environment (FBN). However, studies have shown that this genre is also ighly cosmopolitan in terms of its distribution as a free-living bacterium in ifferent habitats, such as endosphere and rhizosphere of non-leguminous plants. Your ability to ncrease the growth of plants, legumes and non-legumes, put beyond the mechanism f FBN has also been elucidated. In sugar cane, studies using independent methods ultivation, showed that the genus Bradyrhizobium can play an important role in the process BN and growth promotion; which encouraged demand and resulted in the isolation of  genetically diverse collection of bacteria from this genus of sugarcane roots, ome of which had FBN capacity in free life and symbiosis, while others hey had only FBN capacity in free life or symbiosis. Despite reports from the xistence of Bradyziboium as part of the sugarcane microbiome, little is known about he ecology of that interaction. Therefore, the present study aimed to understand the unctionality and ecological effect in the use of bacteria of the genus Bradyrhizobium in the ultivation of sugar cane, as well as, the effect of the practice of crop rotation on the planting of ugarcane in the soil microbial community. The study was divided into four parts, which onsisted of (1) identifying a pattern of differentiation consistent with strains of a radyrhizobium collection, which can be used to guide the identification and selection of few strains through the correlation of phenotypic, genetic and phylogenetic characteristics; (two) understand the double functionality of Bradyrhizobium strain, isolated from the microbiome of sugar cane, in fixing nitrogen in free life and symbiosis; (3) determine the efficiency of strains of Bradyrhizobium spp. to promote growth in sugar cane in greenhouse and field experiment and (4) assess changes in the microbiome sugar cane rhizosphere as a function of crop rotation practice.  The results indicated correspondence between phenotypic characteristics and genetic analyzes and phylogeny from the Bradyrhizobium collection, revealing phenotypic characteristics that differentiate strains in the B. japonicum and B. elkanii supergroups. The presence in Bradyrhizobium of two types of clusters of genes for FBN, one symbiotic (SC) and the other non-symbiotic (NSC), the cluster of non-symbiotic nif genes may be more involved in FBN in free life. In the tests of growth promotion, it was possible to select strains with potential for biotechnological use in sugarcane. In addition, crop rotation with legumes, increased the abundance of rhizobia and other genera of promoter bacteria of growth in the sugarcane rhizosphere.

BANKING MEMBERS:
Presidente - 538.864.458-87 - JOSE IVO BALDANI - UFRRJ
Interno - 1712769 - PEDRO CORREA DAMASCENO JUNIOR
Externo à Instituição - JACKON LEITE - IFPA
Externo à Instituição - ANDRÉ LUIZ MARTINEZ DE OLIVEIRA - UEL
Externo à Instituição - WILLIAN PEREIRA - UFRRJ