Banca de QUALIFICAÇÃO: GUSTAVO TORRES DOS SANTOS AMORIM

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : GUSTAVO TORRES DOS SANTOS AMORIM
DATE: 28/03/2022
TIME: 13:30
LOCAL: UFRRJ (sala virtual)
TITLE:

Selection of periwinkle (Catharanthus roseus (L.) G. Don) genotypes for the production of vincristine: an alkaloid used in the conventional treatment of leukemia and lymphomas

KEY WORDS:

Plant breeding, analysis of secondary metabolites, DNA markers, medicinal plants.

PAGES: 54
BIG AREA: Ciências Agrárias
AREA: Agronomia
SUBÁREA: Fitotecnia
SPECIALTY: Melhoramento Vegetal
SUMMARY:

The periwinkle (Catharanthus roseus (L.) G. Don) produces important alkaloids widely used in cancer treatment, such as vinblastine and vincristine. Vincristine is an alkaloid widely used in the conventional chemotherapy treatment of leukemia and some types of lymphomas. The cost of vincristine extraction from periwinkle plants is very high, as this alkaloid occurs at very low concentrations in the plants. Despite the demand for vincristine in Brazil, the country is not a producer of this alkaloid. Thus, it is extremely important to implement research projects that aim to know the periwinkle genotypes regarding their potential for vincristine production in Brazil. Therefore, this project aimed to know the periwinkle genotypes belonging to the germplasm collection of the UFRRJ Department of Phytotechnics, as to their potential for vincristine production, as well as to know the molecular genetic diversity among promising genotypes. For this purpose, qualitative analyzes to detect presence and absence of total alkaloids in plant leaves will be implemented initially. Dragendroff reagent will be used for detection. The objective is to select from 100 genotypes from the UFRRJ periwinkle germplasm collection those positive for total alkaloid production. Then all these genotypes will be analyzed for their potential for leaf biomass production. The same genotypes will be analyzed by HPLC for vincristine quantification. Genotypes with high vincristine production (high yield and high biomass production) will be subjected to DNA analysis so that crosses can be programmed to obtain superior progenies for vincristine production. All the activities mentioned here will be developed in the periwinkle genetic improvement program developed at UFRRJ since 2017.

BANKING MEMBERS:
Presidente - 1712769 - PEDRO CORREA DAMASCENO JUNIOR
Interna - 2271445 - BRUNA RAFAELA DA SILVA MENEZES
Externa ao Programa - 2147409 - MARILENE HILMA DOS SANTOS
Externo à Instituição - MESSIAS GONZAGA PEREIRA