Productive potential and efficiency of biological nitrogen fixation in elephant grass genotypes and sugarcane varieties.
Biomass; Bioenergy; 15N; Natural Abundance of 15N; BNF
Considering the increase in world energy demand and the environmental impacts generated by the burning of fossil fuels, the search for renewable energy sources to meet this demand has become fundamental. In this context, plant biomass as a source of energy has been gaining ground on the national scene. Elephant grass and sugar cane are two C4 metabolism grasses that have high photosynthetic efficiency and great capacity to accumulate dry matter, which makes them two excellent options for plant species to produce biomass for energy use. The thesis project in question has the objective of: (1) evaluating the productive potential of elephant grass and sugarcane biomass grown in soil poor in Nitrogen (N); (2) quantify the levels of nutrients accumulated in the plants; (3) quantify the efficiency of Biological Nitrogen Fixation (BNF) for nitrogen nutrition in cultures and (4) adjust the methodology with control plants to use the Isotopic Technique for quantifying BNF from the natural abundance of 15N. For this, 2 experiments will be carried out in two concrete tanks with soil marked with 15N and 1 experiment in a greenhouse, in the experimental area of Embrapa Agrobiologia located in the municipality of Seropédica, RJ. The experimental design will be in randomized blocks with four replications. After cutting the plants of experiment I and II, the fresh mass will be weighed and, soon after drying, the dry material will be weighed to obtain the dry matter of the cultures. The material collected will be sampled and analyzed for the levels of macro and micronutrients accumulated in the aerial biomass and the quantification of BNF will be carried out using the method of natural abundance of 15N. Experiment III will be carried out in plastic containers (cups) and with witness plants, after the harvest time it will follow the same methodology described for experiments I and II. The expected results are associated with positive values of N derived from FBN in the biomass production of Elephant grass and Sugarcane crops in order to minimize the economic, energetic and environmental cost by reducing the need for high applications of nitrogen fertilizer, resulting in a positive energy balance of the biomass produced by the two crops under study.