Banca de QUALIFICAÇÃO: GUILHERME SILVA DE ANDRADE

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : GUILHERME SILVA DE ANDRADE
DATE: 08/05/2026
TIME: 14:00
LOCAL: Webconferência
TITLE:

Biological and Ecological Studies of Predatory Lacewing to Optimize Biological Control of Pests in Vegetable Crops

KEY WORDS:

biological control, green lacewing, horticulture, beneficial insectary plants

PAGES: 30
BIG AREA: Ciências Agrárias
AREA: Agronomia
SUBÁREA: Fitotecnia
SUMMARY:

The increase in plant diversity, mainly through the use of plants attractive to natural enemies of insect pests (PAIN), consists of one of the main practices of conservative biological control (CBC), this practice favors, among other factors, the increase in the population of biological control agents (ABC), such as Chrysoperla externa, in this case, the use of PAIN serves as a source of pollen and nectar as insect food in the adult stage (i.e., it is glypolinivorous), and can also serve as food for larvae when there is an absence of prey insects. To investigate the insect's preference in relation to the PAIN species, pollen digestibility in the adult insect, and the spatial distribution of the pest population, three experiments will be set up. Experiment 1 will be conducted in a completely randomized design (DIC), with four factors: pollen species [five levels: diet with sunn hemp pollen (CR), jack bean (FP), millet (MT), corn (MO ) and sorghum (SO)], temperature (three levels: 20 °C, 25 °C and 30 °C), sex (two levels: female and male) and digestion time [14 levels: 0 (positive control), 6 , 12, 18, 24, 48, 72, 96, 120, 168, 264, 336, 384 and 432 h, with two negative controls: adults fed only water and adults fed water + honey (1:1 ratio) ( factorial scheme 5 x 3 x 2 x 14 + 2). Five females and five males of newly emerged C. externa, unmated and fasting for 24 hours will be used for each treatment (1 adult = 1 replicate per sex). In the experiment, 2 bioassays will be conducted in DIC, using the following foods: sunn hemp pollen, jack bean pollen, millet pollen, corn pollen and sorghum pollen and Ephestia kuehniella moth eggs as prey. Each species of pollen will be offered to the larva during one instar and the other two instars will be fed with the prey or with the same pollen, in the following feeding regimes: (a) L1, L2 and L3 with pollen, (b) L1 and L2 with pollen, L3 with prey, (c) L1 and L3 with pollen, L2 with prey (d) L1 with pollen, L2 and L3 with prey, (e) L1 with prey, L2 and L3 with pollen, (f) L1 and L3 with prey, L2 with pollen, and (g) L1 and L2 with prey, L3 with pollen, thus consisting of 7 treatments. Two controls will be included, in one, the three instars will be fed with only the prey (positive control) and in the other, these instars will be without food (SA) (negative control). A minimum of 15 C. externa larvae will be used for each instar (L1, L2 and L3) per treatment. In the third experiment, vegetable cultivation will be carried out with one of the banks cultivated with a strip of PAIN (3 to 4 rows) that will be selected based on data on the preferences of adults and larvae obtained in experiments 1 and 2. Adults samples of C. externa (50% males: 50% females) will be marked with fluorescent dye (Luminous Powder, from Bioquip®) and released in the PAIN range. The margin of vegetable cultivation close to the strip will be considered point 0. Collections of C. externa adults will be carried out 1, 2 and 3 hours after the release of the adults. With this, it is expected that it will be possible to evaluate which PAINs can favor the greatest attraction of insects, and, as the spatial distribution of lacewings in the field occurs, being an important tool to create strategies for the use of this biological control agent (ABC).

COMMITTEE MEMBERS:
Presidente - 2223814 - ELEN DE LIMA AGUIAR MENEZES
Interno - 1863063 - LUIZ AURELIO PERES MARTELLETO
Externa à Instituição - HALINA SCHULTZ
Externa à Instituição - VÂNIA GONÇALVES LOURENÇO ESTEVES