Evaluation methodology for selection of tomato genotypes with partial resistance to fusarium wilt.
Fusarium oxysporum f. sp. lycopersici; Solanum lycopersicum; genetic control.
Tomato fusarium wilt caused by Fusarium oxysporum f. sp. lycopersici (Fol),
can cause high productivity losses and make unfeasible areas for cultivation of the species in
different regions of the world. The pathogen is tomato-specific and may present
physiological specialization to different cultivars. So far three races have been reported
pathogen, and three genes that confer immunity-like resistance to these breeds. THE
tomato genotype evaluation for fol resistance has been done using
methodologies that favor the identification of the selection of accesses with complete resistance in
detriment of partial resistance. The present work aimed to evaluate the methodology
to identify partial resistance genotypes and identify potential accessions
Germplasm Bank of UFRRJ carriers of this type of resistance. Initially
Twelve pre-selected tomato accessions were evaluated for resistance to race 1 and 2 of
Fol. The assay was performed in the moulting phase using three isolates of race 3 of the pathogen.
and the Ponderosa, Mascot, and LA 716 S. pennelli varieties as standard. Then 3 hits
were evaluated to adulthood under greenhouse conditions. In parallel
In assays using seedlings in tubes, three inoculum concentrations were evaluated, 104 105 and 106 microconidia ml-1; and three seedling inoculation methodologies, directly in the soil, blunt root immersion and cut root immersion. In both assays, 3 genotypes and 2 isolates of Fol were used. Inoculations were done at 25 days after sowing and evaluated at 21 days after inoculation. In the seedling assay, variations in the resistance / susceptibility reactions of the accessions were observed as a function of the Fol isolate, with emphasis on the FUS 1405 accession as it presented SU reaction to all isolates used. However, this same result was not observed in the trial until adulthood. It was found that the direct inoculation in soil and immersion of blunt roots at a concentration of 1.0x104 microconidia ml -1 and the use of ENAS 2013 access and cultivar Mascot resulted in a more adequate methodology in the selection of partial resistance genotypes. .