Banca de DEFESA: NATALIA FRASSON LOUREIRO
Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.DISCENTE : NATALIA FRASSON LOUREIRO
DATA : 29/06/2018
HORA: 09:00
LOCAL: PPGF - Sala1
TÍTULO:
Influence of the AAP1 amino acid transporter on general nitrogen metabolism in Arabidopsis thaliana
PALAVRAS-CHAVES:
Amino acids, Arabidopsis thaliana, AtAAP1, Nitrogen metabolism.
PÁGINAS: 33
GRANDE ÁREA: Ciências Agrárias
ÁREA: Agronomia
SUBÁREA: Fitotecnia
ESPECIALIDADE: Fisiologia de Plantas Cultivadas
RESUMO:
It is known that the nitrogen absorbed by plants is assimilated as amino acids, which can also be present in expressive concentrations in some soils and through them to be absorbed directly by the plants. Recent studies have investigated the location and function of amino acid carrier proteins in order to better understand their role in plant metabolism. In order to elucidate the relevance of the AAP1 transporter participation in general nitrogen metabolism in plants, two Arabidopsis thaliana strains genetically transformed for the expression of the AtAAP1 gene were submitted to analysis for the expression of the genes related to the NRT2.1 and NRT1.1 nitrate transporters and AAP6 and AAP8 amino acids , with the goal of investigating the possible effect on their activity under conditions of low external nitrate concentration. For that, an experiment of hydroponic cultivation in phytotron was carried of the Department of Soils of UFRRJ. The experiment had 4 tanks of hydroponics per variety. Each tank received 12 plants and was admitted as a repeat, with 4 in total. The plants were cultured for 45 days in a solution of 10 mM NO3- and 1 mM NH4+. Before collecting, they passed to a solution with no source of N, where they remained for 5 days. At the end of the fifth day they were submitted to a culture solution containing 0.1 mM of NO3- as the only source of N and after 4 hours the collection was performed. At the time of collection, the plants of each hydroponic tank formed a composite sample and were separated in root and shoot. One part was used for analysis of soluble fraction and NR activity and the other was stored in liquid N for later expression analysis of the AtAAP6, AtAAP8, AtNRT1.1, AtNRT2.1 and AtNAR2.1 genes. The results showed that the absence of activity of the AAP1 amino acid transporter is accompanied by the reduction of the activity of two other transporters of the same phylogeny, namely AAP6 and AAP8, as a strategy to avoid the accumulation of amino acids in the root
MEMBROS DA BANCA:
Presidente - 002.180.573-34 - MANLIO SILVESTRE FERNANDES - UFRRJ
Externo à Instituição - CASSIA PEREIRA COELHO BUCHER - UFRRJ
Externo à Instituição - STEFAN SCHWAB - EMBRAPA