Biological fixation and nitrogen transfer using a legume in dwarf plantain cultivation in an organic systemMusa sp, green manure, organic agriculture.
ABSTRACT: The use of tree leguminous species in green manure in plane orchards can be a viable alternative to supply the N demand by fruit trees. Information related to the potential for biological fixation of N2 from the air (BNF) for legumes and its contribution to the supply of N in cultivated areas with orchards is important to assist in choosing the most suitable species for green manure. Therefore, the general objective of this work will be to estimate the BNF and the transfer of N by the methods of the natural abundance of 15N, of the legumes Gliricidia sepium (Jacq) Pers. (gliricidia), in the organic cultivation of plantain, in addition to quantifying the dynamics of decomposition, the production of dry biomass and the supply of nutrients in the system. The experiment will be the use of a tree legume Gliricidia sepium in the green fertilization of plane orchards in a dense system, under organic management, located in the city of Seropédica-RJ. Throughout the experiment, soil analyzes will be carried out in the layers of 0-20 cm depth in the experimental plots, to carry out the analysis of soil fertility, total organic carbon (TOC), granulometric fractionation of SOM and soil physics. These analyzes will be carried out in two distinct stages: the first at the beginning of the experiment and the second at the end of the experiment. The green fertilization will be carried out in the strip cultivation system, in which the pruning will be done in the leguminous bank present in the farm that later the material will be deposited in the projection of the canopy of the fruit trees. To quantify the estimates of FBN and the transfer of fixed N derived from legumes, the techniques of natural abundance of 15N will be used. For the application of isotopic techniques it is necessary to use non-nitrogen fixing plants (control). In this way, two strategies will be carried out, the first one is to collect plants that grew spontaneously among the Gliricidia sepium bank. The second strategy consists of the cultivation of spontaneous plants (non-nitrogen fixing), in the greenhouse of EMBRAPA Agrobiologia, in pots containing samples of 0.5 kg of soil. Soils will be collected at depths of 0.0-0.2, 0.2-0.4 and 0.4-0.6 m, in the legume bank present in the small farm. During the entire experiment, data collection on vegetative and productive characteristics will be carried out in the first production cycle of banana trees. At the time of harvest, two clumps that do not show any visual symptoms of nutrient deficiency and attack by pests and diseases will be selected to sample the plant, which will then be divided into rhizome, pseudostem, petiole, limb, stalk and fruit. Subsequently, the samples will be placed in a greenhouse for pre-drying and then placed in paper bags and taken to a forced air circulation oven at 65ºC, until reaching constant mass. After drying, the samples will be weighed to obtain the accumulation of dry matter. Chemical analyzes of plant tissue will be carried out to determine the accumulation of nutrients in the different parts of the plants, in addition to estimating the absolute and relative export of nutrient by the bunches and the stalk. At the end of this research, green manure with legumes is expected to provide N to reduce the use of organic fertilizers in plantain crops, maintaining productivity and the nutritive value of the fruits, as well as reducing production costs, in addition to providing viable strategies for the family farmers in Rio de Janeiro, to increase productivity, reduce costs with crop management.