Banca de QUALIFICAÇÃO: ANDRÉ LUÍS DA SILVA NOGUEIRA

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : ANDRÉ LUÍS DA SILVA NOGUEIRA
DATE: 19/09/2022
TIME: 09:00
LOCAL: VIDEOCONFERÊNCIA
TITLE:

Characterization of biotechnological targets involved in drought tolerance in rice

KEY WORDS:

 Water stress,  Genes, Biotechnology, CRISPR/Cas9.

PAGES: 30
BIG AREA: Ciências Agrárias
AREA: Agronomia
SUBÁREA: Ciência do Solo
SUMMARY:

As it is the abiotic stress with the greatest effect on plants, drought is a key factor to be studied to avoid restricting the development of agriculture. In the plant, it affects from photosynthesis, respiration and cellular metabolism, impacting growth and production. Despite this, plants, sessile organisms, have developed mechanisms that lead them to tolerate the effects of drought, modulating their genetic machinery, leading to the expression of genes that code for proteins that allow survival in water restriction. From this perspective, there are genes that still do not have their function characterized and there is a need to understand about them, and the objective of this project is to identify and characterize regulatory genes with the potential to increase drought tolerance in rice. For this, research is being carried out at the Laboratory of Mineral Nutrition of Plants (LNMP) of the Soil Department of the Federal Rural University of Rio de Janeiro (UFRRJ). Thereby, two genes with the potential to regulate tolerance to water scarcity in rice plants will be selected. For the gene characterization process, expression cassettes with the knocked out or overexpressed genes will be assembled. The transformation of rice plants (Oryza Sativa), cultivar Nipponbare, will be mediated by Agrobacteirum tumefaciens. After obtaining the mutant plants, lineage selection will be carried out, and then evaluations regarding productivity components and physiological variables, root development, expression of marker genes for water stress and cellular sublocalization of both genes.

BANKING MEMBERS:
Externa à Instituição - ROSILENE OLIVEIRA MESQUITA - UFC
Interno - 2213075 - EVERALDO ZONTA
Presidente - 2626406 - LEANDRO AZEVEDO SANTOS
Externa à Instituição - MARCIA SOARES VIDAL - EMBRAPA