Banca de DEFESA: NATÁLIA DOS SANTOS FERREIRA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : NATÁLIA DOS SANTOS FERREIRA
DATE: 29/11/2022
TIME: 09:00
LOCAL: Defesa com apresentação via googlemeet
TITLE:

TAXONOMY AND RECLASSIFICATION OF STRAINS OF THE GENUS AZOSPIRILLUM AND NITROSPIRILLUM BASED ON PHYLOGENY, GENOMICS AND FUNCTIONAL ATTRIBUTES

KEY WORDS:

Culture collection, new species, taxonomic reclassification, Azospirillum, Nitrospirillum.

PAGES: 120
BIG AREA: Ciências Agrárias
AREA: Agronomia
SUBÁREA: Ciência do Solo
SPECIALTY: Microbiologia e Bioquímica do Solo
SUMMARY:

The Johanna Döbereiner Biological Resource Center - CRB-JD, is a culture collection belonging to the National Center for Research in Agrobiology (CNPAB), officially exists since 1960, is world-renowned for maintaining a high diversity of nitrogen-fixing bacteria, in addition to containing microbial species that have the potential to be used for the benefit of Brazilian society. Today its collection houses about (53) yeasts, (64) filamentous fungi, (6060) bacteria of different genera. Much of the Collection was identified using biochemical and morphological features. However, taxonomic information on the strains is still lacking, based on currently accepted molecular methods in genome analysis. In view of this, the present work proposed, through molecular analysis, to characterize a part of the collection of its germplasm collection for the genus Azospirillum and Nitrospirillum. Both genera have plant growth-promoting rhizobacteria with the ability to fix atmospheric nitrogen and produce beneficial compounds for plants and are present in different soil and climatic conditions. Molecular analyzes were based on combined phylogenetic characterizations (16S rRNA, use of housekeeping genes); genomics (average nucleotide identity (ANI) with circumscription values of 96% and DNA-DNA digital hybridization (dDDH) cut-line of 70%, core proteome). Functional analyzes defined only for Azospirillum species, which are based on phenotypic and biochemical characterizations. This work originated two taxonomic reclassification proposals, defined as Azospirillum baldaniorum Sp245T and Azospirillum argentinense Az39T, previously classified as species belonging to Azospirillum brasilense. In addition to the phylogenetic classification of strains of the genus Azospirillum and Nitrospirillum, evidencing the high diversity between strains of the species of both genera, suggesting the existence of new species and adding information to the database of this collection of wrongly classified strains belonging to other genera.

COMMITTEE MEMBERS:
Presidente - ***.881.579-** - JERRI ÉDSON ZILLI - EMBRAPA
Interna - 1815763 - IRENE DA SILVA COELHO
Interna - ***.052.847-** - VERONICA MASSENA REIS - EMBRAPA
Externa à Instituição - EVELISE BACH
Externa à Instituição - FÁTIMA MARIA DE SOUZA MOREIRA