Symbiotic efficiency of Bradyrhizobium strains inoculated in cowpea and soybean.
Bradyrhizobium,, cowpea and soybean
The establishment of symbiosis between legumes and nitrogen-fixing bacteria
atmosphere can increase productivity and reduce production costs, since the
N is the nutrient most required by plants and the use of nitrogen fertilizers
aggravates environmental problems, in addition to generating high production costs. In the present
study, a cross-inoculation experiment was carried out in a greenhouse with
50 strains of Bradyrhizobium isolated from the experimental field at Embrapa
Agrobiology, in the municipality of Seropédica inoculated in cowpea and soybean.
Subsequently, an experiment of symbiotic efficiencies was carried out with the strains
selected from the previous experiment and compared with strains recommended by the
MAP for cowpea and soybean inoculation, through the evaluation of the following
parameters: number of nodules, dry mass of nodules and dry mass of shoots.
In addition to the greenhouse experiments, the relationships
phylogenetics of strains through amplification and sequencing of 16S genes
rDNA, recA and nodC. It was demonstrated that 30 strains nodulated the cowpea,
indicating a good performance in biological nitrogen fixation and that only 11
strains established symbiosis with the soybean cultivar, demonstrating that this legume
has greater host specificity.