Banca de DEFESA: ERINALDO GOMES PEREIRA
Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.DISCENTE : ERINALDO GOMES PEREIRA
DATA : 27/02/2019
HORA: 09:00
LOCAL: Sala 24 Departamento de Solos do Instituto de Agronomia da UFRRJ
TÍTULO:
Obtaining of rice mutants for the gene OsAAP1 using the CRISPR- Cas 9 system for the identification of functions associated to nitrogen use efficiency (NUE)
PALAVRAS-CHAVES:
Amino acid carrier.Piauí variety.Oryza sativa L.
PEREIRA, Erinaldo Gomes. Obtaining of rice mutants for the gene OsAAP1 using the CRISPR- Cas 9 system for the identification of functions associated to nitrogen use efficiency (NUE). 2019. 49p. Dissertation (Master Science in Agronomy, Soil Science). Instituto de Agronomia, Departamento de Solos, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, 2019.
The integration of acquired knowledge on the processes of N-inorganic regulation, transport and metabolism, with the study of N-organic, using modern tools such as the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) system, is one of the paths for optimization production processes and can contribute to the development of more sustainable agriculture. The main objectives of this work were to verify the role of the transporter of amino acids OsAAP1 (Amino acid Permease 1) of rice (Oryza sativa L.) in the initial development of the plants, its influence on inorganic and organic N transporters, possible alterations in the expression of genes that encode key enzymes for carbon metabolism, as well as the performance of this transporter in grain production and in the use of nitrogen (NUE). For this, the OsAAP1 gene was knockout in plants of the Piauí variety using the CRISPR-Cas9 system and two lines were selected for the study (L3 and L5). Experiments 1, for metabolic characterization and study of the expression of genes involved in N-inorganic transport and in carbon metabolism, were conducted in the experimental chamber of the Federal Rural University of Rio de Janeiro (UFRRJ), in nutrient solution of Hoagland and Arnon (1950), with 2 mM and 0.2 mM of N-nitric (KNO3-) as the only source of N for 40 days. Experiment 2, for metabolic characterization, study of the expression of genes involved in N-organic transport and carbon metabolism, determination of production parameters and efficiency in the use of N, was conducted in the greenhouse of the Laboratory of Mineral Nutrition of Plants (LNMP) in pots containing soil from a horizon A of a planosol collected at the UFRRJ campus. A basal fertilization, at 30 days after germination, was performed, equivalent to 60 kg / ha of N-nitric (KNO3-). At the end of the study it was possible to verify that the AAP1 transporter is involved in the internal flow of N in rice plants, and the loss of function of this transporter negatively interferes in the processes of absorption and assimilation of inorganic N, causing a regulation in the expression of genes that code for nitrate transporters, amino acids and carbon cycle enzymes, and lead to a decrease in grain yield and EUN.
PÁGINAS: 88
GRANDE ÁREA: Ciências Agrárias
ÁREA: Agronomia
SUBÁREA: Ciência do Solo
ESPECIALIDADE: Microbiologia e Bioquímica do Solo
RESUMO:
PEREIRA, Erinaldo Gomes. Obtaining of rice mutants for the gene OsAAP1 using the CRISPR- Cas 9 system for the identification of functions associated to nitrogen use efficiency (NUE). 2019. 49p. Dissertation (Master Science in Agronomy, Soil Science). Instituto de Agronomia, Departamento de Solos, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, 2019.
The integration of acquired knowledge on the processes of N-inorganic regulation, transport and metabolism, with the study of N-organic, using modern tools such as the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) system, is one of the paths for optimization production processes and can contribute to the development of more sustainable agriculture. The main objectives of this work were to verify the role of the transporter of amino acids OsAAP1 (Amino acid Permease 1) of rice (Oryza sativa L.) in the initial development of the plants, its influence on inorganic and organic N transporters, possible alterations in the expression of genes that encode key enzymes for carbon metabolism, as well as the performance of this transporter in grain production and in the use of nitrogen (NUE). For this, the OsAAP1 gene was knockout in plants of the Piauí variety using the CRISPR-Cas9 system and two lines were selected for the study (L3 and L5). Experiments 1, for metabolic characterization and study of the expression of genes involved in N-inorganic transport and in carbon metabolism, were conducted in the experimental chamber of the Federal Rural University of Rio de Janeiro (UFRRJ), in nutrient solution of Hoagland and Arnon (1950), with 2 mM and 0.2 mM of N-nitric (KNO3-) as the only source of N for 40 days. Experiment 2, for metabolic characterization, study of the expression of genes involved in N-organic transport and carbon metabolism, determination of production parameters and efficiency in the use of N, was conducted in the greenhouse of the Laboratory of Mineral Nutrition of Plants (LNMP) in pots containing soil from a horizon A of a planosol collected at the UFRRJ campus. A basal fertilization, at 30 days after germination, was performed, equivalent to 60 kg / ha of N-nitric (KNO3-). At the end of the study it was possible to verify that the AAP1 transporter is involved in the internal flow of N in rice plants, and the loss of function of this transporter negatively interferes in the processes of absorption and assimilation of inorganic N, causing a regulation in the expression of genes that code for nitrate transporters, amino acids and carbon cycle enzymes, and lead to a decrease in grain yield and EUN.
PEREIRA, Erinaldo Gomes. Obtaining of rice mutants for the gene OsAAP1 using the CRISPR- Cas 9 system for the identification of functions associated to nitrogen use efficiency (NUE). 2019. 49p. Dissertation (Master Science in Agronomy, Soil Science). Instituto de Agronomia, Departamento de Solos, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, 2019.
The integration of acquired knowledge on the processes of N-inorganic regulation, transport and metabolism, with the study of N-organic, using modern tools such as the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) system, is one of the paths for optimization production processes and can contribute to the development of more sustainable agriculture. The main objectives of this work were to verify the role of the transporter of amino acids OsAAP1 (Amino acid Permease 1) of rice (Oryza sativa L.) in the initial development of the plants, its influence on inorganic and organic N transporters, possible alterations in the expression of genes that encode key enzymes for carbon metabolism, as well as the performance of this transporter in grain production and in the use of nitrogen (NUE). For this, the OsAAP1 gene was knockout in plants of the Piauí variety using the CRISPR-Cas9 system and two lines were selected for the study (L3 and L5). Experiments 1, for metabolic characterization and study of the expression of genes involved in N-inorganic transport and in carbon metabolism, were conducted in the experimental chamber of the Federal Rural University of Rio de Janeiro (UFRRJ), in nutrient solution of Hoagland and Arnon (1950), with 2 mM and 0.2 mM of N-nitric (KNO3-) as the only source of N for 40 days. Experiment 2, for metabolic characterization, study of the expression of genes involved in N-organic transport and carbon metabolism, determination of production parameters and efficiency in the use of N, was conducted in the greenhouse of the Laboratory of Mineral Nutrition of Plants (LNMP) in pots containing soil from a horizon A of a planosol collected at the UFRRJ campus. A basal fertilization, at 30 days after germination, was performed, equivalent to 60 kg / ha of N-nitric (KNO3-). At the end of the study it was possible to verify that the AAP1 transporter is involved in the internal flow of N in rice plants, and the loss of function of this transporter negatively interferes in the processes of absorption and assimilation of inorganic N, causing a regulation in the expression of genes that code for nitrate transporters, amino acids and carbon cycle enzymes, and lead to a decrease in grain yield and EUN.
MEMBROS DA BANCA:
Externo à Instituição - ALTAMIRO SOUZA DE LIMA FERRAZ JUNIOR - UEMA
Presidente - 002.180.573-34 - MANLIO SILVESTRE FERNANDES - UFRRJ
Externo ao Programa - 1736993 - SILVIA APARECIDA MARTIM