Banca de DEFESA: MARCELA JACQUES DE LEMOS NEVES
Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.DISCENTE : MARCELA JACQUES DE LEMOS NEVES
DATA : 28/02/2019
HORA: 09:00
LOCAL: Sala Marcelo Nunes Camargo, n°24, Departamento de Solos
TÍTULO:
Phenotypic and metabolic characterization of mutant rice strains silencing H + ATP isoforms responsive to nitrogen supply 2019
PALAVRAS-CHAVES:
H+-ATPase, nitrogen, Oryza sativa
PÁGINAS: 72
GRANDE ÁREA: Ciências Agrárias
ÁREA: Agronomia
SUBÁREA: Ciência do Solo
ESPECIALIDADE: Microbiologia e Bioquímica do Solo
RESUMO:
JACQUES, Marcela de Lemos Neves. Phenotypic and metabolic characterization of mutant rice strains silencing H + ATP isoforms responsive to nitrogen supply 2019. 60p. Thesis (Doctor Science in Agronomy - Soil Science). Instituto de Agronomia, Departamento de Solos, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ. 2019.
In rice, there are ten H + - ATPase isoforms, and the most expressed isoforms are the OsA2 and OsA7 isoforms, in addition to being induced through the supply of NO 3 - . At PM H + ATPases , besides having great importance in the absorption of nutrients, also plays a fundamental role in several biological processes in plants. Studies with silicon isoforms of PM H + - ATPase in rice at different doses of nitrogen were detrimental to vegetative growth and grain yield. Therefore, one of the objectives of this work was to evaluate the effect of silencing on phenotypic characteristics, grain yield and protein content in the grain of the rice strains under different nitrate (NO 3 - ) availability conditions . The experiment was conducted in a greenhouse until the end of the rice crop cycle, using untransformed plant (WT), plant with the silenced osa2 isoform and plants with silica isoform osa7, which were submitted to treatment with NO 3 - and without NO 3 - resupply . The height of the plants, number of tillers, production of full grains and pips, harvest index, number of panicles were evaluated. According to the results, it can be assumed that the silencing of the isoforms affected the vegetative development of the osa7 and osa2 mutant plants , indicating that PM H + - ATPase activity may have affected some cellular process, resulting in reduced plant growth , besides exerting influence in the filling of the grains. Among the stresses that plants undergo in the environment saline stress considerably influences the growth and productivity of large crops. Several studies have demonstrated that treatments with the presence of salt in the plants, caused the activity of the proton pump of the plasma membrane. In a study related to the addition of NaCl , they observed an increase in transcription levels of the OsA2 isoform. It was also observed that the OsA7 isoform may be related to salinity tolerance. Therefore, the objective of this work was to evaluate the effect of salinity on rice plants with silica osa2 and osa7 isoforms, on nitrogen absorption and plant utilization.According to the results it can be affirmed that in the transformed lines there was a reduction of the growth of these plants , as well as accumulation of NO 3 - mainly in the roots, in relation to the N-amino results there is an accumulation in the leaf . According to the results, it is shown that PM H + - ATPases isoforms have great importance in saline stress, since the silencing of OSA2 and OSA7 isoforms affected the development and efficiency of NO 3 - and stomatal conductance .
MEMBROS DA BANCA:
Externo ao Programa - 2873182 - DIEGO DE MELLO CONDE DE BRITO
Externo ao Programa - 2333129 - FABIOLA DE SAMPAIO RODRIGUES GRAZINOLI GARRIDO
Presidente - 2626406 - LEANDRO AZEVEDO SANTOS
Externo à Instituição - LUIZ MORS CABRAL - UFF
Externo à Instituição - MARCIO DOS REIS MARTINS - EMBRAPA