Banca de DEFESA: LUANA SOUSA COSTA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : LUANA SOUSA COSTA
DATA : 07/08/2018
HORA: 09:00
LOCAL: Sala 24 Departamento de Solos do Instituto de Agronomia da UFRRJ
TÍTULO:

Simultaneus growth of Paraburkholderia tropica (strain BR 11366) and Gluconacetobacter diazotrophicus (BR 11281) for the production of a bacterial inoculant.

PALAVRAS-CHAVES:

Bioprocess, sugarcane, diazotrophic bacteria, growth promotion.

PÁGINAS: 70
GRANDE ÁREA: Ciências Agrárias
ÁREA: Agronomia
SUBÁREA: Ciência do Solo
ESPECIALIDADE: Microbiologia e Bioquímica do Solo
RESUMO:

A mixed inoculant was proposed for sugarcane in 2008 based on long-term studies of inoculation and performance of diazotrophic bacteria. However, this product is not available to producers due to some technical factors, such as insufficient development of bioprocesses for the industrial multiplication of microorganisms. The efficiency and productivity of the bioprocesses are important components to enable the production of the inoculant on an industrial scale. Because of this, the production strategy goes beyond the process of choosing the appropriate microorganisms. Parameters associated with growth were tested to establish the maximum biomass production of cells by simultaneous cultivation of two strains comprising the mixed inoculant, Paraburkholderia tropica (BR 11366T strain) and Gluconacetobacter diazotrophicus (BR 11281T), for the production of commercial inoculants. The strains have quite distinct growth under the conditions tested. The maximum biomass production in simultaneous culture (quantified by real-time PCR) was observed in a bench-fed bioreactor, aerated at 4 L min-1 and temperature at 32 ° C, reaching 8.73 · 108 CFU mL -1 and 5.05 · 1010 UFC mL -1 for BR 11281 and BR 11366 respectively. In this way, cells with these two strains can be obtained by simultaneous culturing.

MEMBROS DA BANCA:
Presidente - 631.052.847-53 - VERONICA MASSENA REIS - UFRRJ
Interno - 1815763 - IRENE DA SILVA COELHO
Externo ao Programa - 2136627 - NIVALDO SCHULTZ
Externo à Instituição - MARCIA SOARES VIDAL - UFRJ
Externo à Instituição - LEDA MARIA FORTES GOTTSCHALK - UFRJ