Banca de DEFESA: NATHALIA DE FRANÇA GUIMARÃES
Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.DISCENTE : NATHALIA DE FRANÇA GUIMARÃES
DATA : 28/02/2020
HORA: 09:00
LOCAL: Sala Multimeios da Embrapa Agrobiologia
TÍTULO:
Identification and growth promotion of diazotrophic bacteria isolated from different genotypes of the Saccharum genus.
PALAVRAS-CHAVES:
Complex Saccharum. Diazotrophs. Plant-bracteria interaction. Bacterium isolation. rRNA
PÁGINAS: 115
GRANDE ÁREA: Ciências Agrárias
ÁREA: Agronomia
SUBÁREA: Ciência do Solo
ESPECIALIDADE: Microbiologia e Bioquímica do Solo
RESUMO:
Bacteria described as plant growth promoters can act on the development of sugarcane cultivars through various mechanisms, such as biological nitrogen fixation, nutrient solubilization, synthesis of siderophores and plant growth hormones, in addition to contributing to the resistance of biotic stresses and abiotic from different plants. Therefore, this study aimed to evaluate the growth promotion of inoculated diazotrophic bacteria in cultivars belonging to the Saccharum complex, and from root samples, isolate and characterize bacteria from these different genotypes. Several experiments were carried out in a greenhouse using the cultivars: one belonging to the species Saccharum spontaneum - US72-1319, five from Erianthus arundinaceus - IJ76-364, IJ76-358, IJ76-359, IJ76-381 and IJ76-384; two from Saccharum robustum - IJ76-414 and IM76-228, two from Saccharum barberi - White Pararia and IN84-58, one Saccharum sinense - Maneria, three Saccharum officinarum - White, Biscuit and Q45830 and one Miscanthus sp. - cultivate Fiji 15; all from Embrapa's germplasm bank. Experiments were developed with a randomized block design (DBC) in a double factorial scheme (2x2) with five replications. The factors were: absence or presence of inoculation with five strains of diazotrophic bacteria: Gluconacetobacter diazotrophicus; Herbaspirillum seropedicae; Herbaspirillum. rubrisubalbicans; Paraburkholderia tropica and Nitrospirillum amazonense; and absence or not of short thermal treatment (TT) (52°C for 30 min). The mini-specimens (one yolk) were placed in a box containing sterile substrate to evaluate sprouting, in addition to biometric growth parameters and analysis of root architecture using WinRhizo Pro® software. From the root tissue, 88 bacterial strains were isolated, forming 18 clusters using morphological characters. Representatives of these groups were evaluated for phylogenetic identification by sequencing the 16S in addition to physiological characteristics of growth promotion. The results of sprouting speed index and number of sprouts show that thermotherapy affects all species studied, with the exception of the species IJ76-358, IJ76-364, US72-1319, which presented neutral sensitivity. The inoculation positively affected these variables the species IJ76-315, IJ76-414, Biscuit, Branca and IM76-228 when in the absence of TT and Bamboo Rose in the presence of TT. Among the analyzed sequences, two groups belonging to the phyla Proteobacteria and Firmicutes were identified and distributed in 16 genera. The results suggest that the selected bacteria have biotechnological potential to promote plant growth
MEMBROS DA BANCA:
Presidente - 631.052.847-53 - VERONICA MASSENA REIS - UFRRJ
Interno - 1104775 - ADELSON PAULO DE ARAUJO
Externo à Instituição - LUC FELICIANUS MARIE ROUWS - EMBRAPA
Externo à Instituição - LUIS HENRIQUE DE BARROS SOARES - EMBRAPA
Externo à Instituição - WILLIAN PEREIRA - UFRRJ