Banca de DEFESA: RENAN PINTO BRAGA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : RENAN PINTO BRAGA
DATE: 30/10/2020
TIME: 09:00
LOCAL: Webconferência
TITLE:

Functional characterization of the OsDof26 transcription factor through genetic knockout and analysis of the metabolic profile in rice

KEY WORDS:

Nitrogen. Transcription factor. CRISPR. Dof. Metabolism.

PAGES: 100
BIG AREA: Ciências Agrárias
AREA: Agronomia
SUBÁREA: Ciência do Solo
SPECIALTY: Fertilidade do Solo e Adubação
SUMMARY:

Nitrogen and Carbon metabolism are key parts in improving food production, which needs to increase to maintain the diet of the growing human population.
Transcription factors are regulatory proteins of gene expression and can be key elements in the control of intricated metabolic pathways. DNA Binding with One Finger (Dof) Transcription Factors are unique to green plants and are directly related to metabolic events unique to the plant kingdom. This study aimed to verify the probable function of the OsDof26 Transcription Factor through gene knockout using CRISPR-Cas9 technology and its possible influence on nitrogen and carbon metabolism. The results obtained show that the absence of this Transcription Factor harmed the rice plant, reducing its production of biomass and grains. There was also the accumulation of carbohydrates necessary for primary carbon metabolism and nitrogen assimilation in roots.

BANKING MEMBERS:
Externo ao Programa - 1809123 - ANDRE MARQUES DOS SANTOS
Interno - 1399485 - ANDRES CALDERIN GARCIA
Externo à Instituição - LUIZ MORS CABRAL - UFF
Presidente - 002.180.573-34 - MANLIO SILVESTRE FERNANDES - UFRRJ
Externa à Instituição - RAFAELA ELOI DE ALMEIDA ALVES