Grain Yield of Mung bean (Vigna radiata (L.) Wilczek) inoculated with strain BR 3302 from cowpea (V. unguiculata (L.) Walp) in the municipality of Campos de Goytacazes - RJ
Vigna radiata (L.) Wilczek, Bradyrhizobium, Biological Nitrogen Fixation, Plant Growth Promotion Bacteria.
Mung beans (Vigna radiata (L.) Wilczek), originally from India, are part of the dietary base of several tropical and subtropical countries due to their high protein value, characterizing a high quality pulse and can be an alternative for the diversification of grain-producing legumes in Brazil. The aim of the present study was to evaluate the effect of inoculation of mung bean seeds with Bradyrhizobium strains registered for cowpea (V. unguiculata (L.) Walp), soybean (Glycine max L.) and common bean (Phaseolus vulgaris L.) on grain yield. A trial under axenic conditions was previously installed in a greenhouse at Embrapa Agrobiologia, Seropédica-RJ, for the selection of efficient strains for mung beans, with twelve treatments (4 strains from cowpea, 4 from soybean, 3 from common bean and an uninoculated control). The variables analyzed were: number of nodules (NN), accumulated dry mass of nodules (NDM), root (RDM) and shoot (SDM). Three cowpea strains (BR 3301, BR 3302 and BR 3267) and one soybean strain (BR 96) were able to form nodules in mung beans and promote biological nitrogen fixation (BNF). Simultaneously, a test was performed with increasing levels of N, aiming at determining the optimal dose to be applied under field conditions as a positive control. From the results obtained for the analyzed variables (RDM and SDM), an optimal dose of 240 kg of N ha-1 was estimated. A trial with six treatments (4 selected strains, uninoculated control and application of 240 kg of N ha-1) and four replications was installed in the UFRRJ experimental field, Campos dos Goytacazes-RJ. The variables analyzed were: NN, NDM, RDM, SDM, accumulated N and N content in the aerial part and grain yield. The variables associated with BNF determined by the 15N natural abundance technique were also evaluated: N content derived from BNF, atmospheric N accumulated (Ndfa) and nodular efficiency (Ndfa NN-1). Yield of mung bean grains inoculated with strain BR 3302 was about 18% more than the non-inoculated control and did not differ significantly from the treatment that received 240 kg N ha-1. However, as BNF contribution by BR 3302 strain was similar to the control, the yield increase resulted mainly from soil N, suggesting that this strain has other promoting plant growth characteristics in addition to BNF. Under the experimental conditions characterized by a naturalized bacterial community capable of providing around 40% of the N for the crop, in addition to the presence of available N in the soil, BR 3302 through growth promotion activity provided 1834 kg of grains ha-1 derived from soil N and 1003 kg of grains ha-1 derived from BNF. The versatility of BR 3302 strain may indicate an adaptive potential capable to meet the demand of mung bean crop under different edaphic and climatic conditions.