Banca de DEFESA: LORRAINE CRISTINA HENRIQUE ALMEIDA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : LORRAINE CRISTINA HENRIQUE ALMEIDA
DATE: 18/12/2020
TIME: 09:00
LOCAL: videoconferência
TITLE:

Thermo-biochemical control of rickets in the stalk of pre-sprouted sugarcane seedlings. 

KEY WORDS:

Sugarcane. Thermotherapy. Diazotrophic bacteria. Leifsoniaxylisubsp. xyli.

PAGES: 50
BIG AREA: Ciências Agrárias
AREA: Agronomia
SUMMARY:

In the culture of sugarcane, heat treatment is used to control an important disease, Raquitismo da Soqueira, caused by the pathogenic bacteria Leifsoniaxyli subsp. Xyl (Lxx). To reduce the inoculum of such a pathogen in the production of pre-sprouted seedlings, heat treatment is used in the mini-jackets with water at 52 ° C for 30 min. However, studies show that such treatment has leaks that serve as a source of inoculum. Among the beneficial effects of plant growth-promoting bacteria, BPC’s, it is believed that the disease-suppressing effect may assist in the biocontrol of Leifsoniaxyli subsp. Xyli for competing for colonization sites, since both colonize xylem vessels. The objective of the present work was to improve the thermal treatment with the addition of chemical additive to the 52 ° C treatment, together with the inoculation with plant growth promoting bacteria, which allows greater efficiency in the control of Lxx, little interference in sprouting and less time of thermotherapy and evaluate: sprouting speed index (IVB), survival of diazotrophic bacteria by the Most Probable Number Method (NMP), biological N2 fixation by the acetylene reduction technique (ARA) and quantify the effect of the treatments used in the control of Lxx through qPCR under the hypothesis that thermotherapy with reduced time and with chemical additive using 2% acetic acid may favor the initial establishment of diazotrophic bacteria used as a sugarcane inoculant and have a biocontrol effect on the causative pathogen of rickets of the brass knuckle. The treatments used were: Heat treatments with water at 52 ° C for 30 "; acetic acid solution 2%; without heat treatment or additive (control); Uninoculated treatment (control); inoculated with the mixture of 5 strains Nitrospirillum amazonense, Herbaspirillum seropedicae, Herbaspirillum rubrisubalbicans, Paraburkholderia tropica, Gluconacetobacter diazotrophicus.

BANKING MEMBERS:
Externo à Instituição - JEAN LUIZ SIMÔES DE ARAÚJO - EMBRAPA
Interno - 2626406 - LEANDRO AZEVEDO SANTOS
Externo ao Programa - 2136627 - NIVALDO SCHULTZ
Presidente - 631.052.847-53 - VERONICA MASSENA REIS - EMBRAPA