Banca de DEFESA: THAINÁ LOUZADA DOS SANTOS
Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.STUDENT : THAINÁ LOUZADA DOS SANTOS
DATE: 31/08/2022
TIME: 09:00
LOCAL: Defesa Online (plataforma Google meet)
TITLE:
Influence of Structural Characteristics and Source of Humified Organic Matter on Growth and Development of Basidiomycete Fungi Pleurotus eryngii, Panus strigellus (Berk.) and Ganoderma lucidum
KEY WORDS:
Humified organic matter; Mushrooms; Chemical characterization, ligno-cellulosic substrates
PAGES: 110
BIG AREA: Ciências Agrárias
AREA: Agronomia
SUBÁREA: Ciência do Solo
SPECIALTY: Microbiologia e Bioquímica do Solo
SUMMARY:
Basidiomycete fungi such as: Pleurotus eryngii, Panus strigellus (Berk.) and Ganoderma lucidum have key properties in nature. A lot of them are decomposers of ligno-cellulosic organic substrates, others have medicinal and pharmacological properties. These basideomycete fungi are key to nutrient cycling, especially organic carbon, and nitrogen, in diverse ecosystems, especially in tropical climates. However, the relationship between the structural quality of organic matter, specifically the carbon types of humified organic matter, and the growth of the fungi Pleurotus eryngii, Panus strigellus (Berk.) and Ganoderma lucidum is poorly understood. This thesis aimed to evaluate the relationship between the growth and development of the basidiomycetes Pleurotus eryngii, Panus strigellus (Berk.) and Ganoderma lucidum in two well-characterized structurally different sources of humified organic matter. The growth and development (mycelial diameter, mycelial run, mycelium fresh and dry mass, mycelial density, and biological yield) of the fungi were evaluated in experiments with application in solid culture medium of humic substances (SH) extracted from vermicompost (VC) and peat. Both SH were characterized by 13C NMR CP/MAS spectroscopy combined with chemometrics (principal component analysis -PCA and multivariate resolution curve -MCR). The HS applied to the fungus have different structural characteristics, where those extracted from VC have a predominance of functionalized C-aliphatics belonging to carbohydrates, low hydrophobicity, and a proportion of 90% of carbon cellulose/hemicellulose in the composition, in contrast, the HS extracted from peat have predominance of C-aromatics corresponding to ligninic fragments, greater hydrophobicity and a proportion of ligninic carbon up to 80%. The results showed that the growth of the basidiomycete fungi Pleurotus eryngii, Panus strigellus (Berk.) and Ganoderma lucidum is dependent on the C-cellulosic and C-lignin balance. The HS extracted from highly ligninic peat regulate the growth of the fungus in the initial moments and at times inhibit the biological performance. In contrast, the highly cellulosic VC HS regulate fungal growth at later times as well as its biological performance. In this thesis, results were obtained that are important because they serve as a basis for understanding the relationship between basidiomycete fungi and the quality of necromass in tropical environments. At the same time, these results may contribute to improving the base of organic substrates in the production of edible and medicinal mushrooms.
BANKING MEMBERS:
Presidente - 1399485 - ANDRES CALDERIN GARCIA
Interno - 387406 - RICARDO LUIZ LOURO BERBARA
Externo ao Programa - 387274 - PAULO SERGIO TORRES BRIOSO - UFRRJExterna à Instituição - CAMILA PINHEIRO NOBRE - UEMA
Externo à Instituição - WALLACE LUIS DE LIMA - IFES