Banca de DEFESA: NATHALIA SOARES CAMARGO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : NATHALIA SOARES CAMARGO
DATE: 17/11/2023
TIME: 13:30
LOCAL: Instituto de Química, sala 49 ou 50
TITLE:

Evaluation of antioxidante potential of unconventional food plant extract Xanthosoma sagittifolium L. Schott at Saccharomyces cerevisiae cells

KEY WORDS:

Antioxidant; PANC; Saccharomyces cerevisiae.

PAGES: 74
BIG AREA: Ciências Exatas e da Terra
AREA: Química
SUMMARY:

Unconventional Food Plants is the term used to refer to a group of plants that have one or more edible parts, whether native or cultivated, and are not part of the daily diet of the population. An example of this is Xanthosoma sagittifolium L. Schott (taro), where studies point to a range of potential activities attributed to this plant, such as anti-inflammatory, antidiabetic, and antioxidant properties. The chemical characterization of Xanthosoma sagittifolium L. Schott describes the presence of flavonoids. Therefore, when introduced into the diet, it can assist the body in maintaining cellular redox homeostasis, helping to prevent oxidative stress, which is a condition caused by the inability of the antioxidant defense system to control the action of oxidants in cells, which can lead to various damages. However, it is important to delve more deeply into the compounds present in this plant and to analyze in more detail the influence of its antioxidant activity using a biological model that takes into account physiological conditions and metabolic interactions. Thus, the toxicity and antioxidant potential of the aqueous extract of X. sagittifolium were evaluated through cell viability assays, mitochondrial functionality, lipid peroxidation, and intracellular oxidation levels. Two strains of S. cerevisiae were used in this study, one control and one with a deletion in the YAP1 gene, a transcription factor for antioxidant defenses in yeast. The chemical profile of the aqueous extract of X. sagittifolium was verified by HPLC-DAD. All analyzed concentrations of the extract in both strains were not found to be toxic. The analysis of the effect of the extract on cell viability revealed that pretreatment with the extract at a concentration of 0.6 mg L^-1 can provide protection for both strains against the stressor used (H₂O₂) at a concentration of 1.0 mM. In antioxidant assays, for the control strain, the X. sagittifolium extract is capable of providing antioxidant protection to the cells. In contrast, the same was not observed in the mutant strain, where only in viability antioxidant assays was the X. sagittifolium extract able to provide protection to the cells, while in the other antioxidant assays, the strain exhibited a higher degree of sensitivity to oxidative stress. The chemical profile of the extract indicates the presence of flavonoids, accounting for 15 of the 17 peaks obtained in the chromatogram. In this study, it was indicated that the extract was non-toxic at the tested concentrations and exhibited antioxidant properties. These antioxidant properties can be attributed to the presence of flavonoids in the extract, suggesting that taro has the potential to be a promising source of compounds with antioxidant action.

COMMITTEE MEMBERS:
Presidente - 1844240 - CRISTIANO JORGE RIGER
Interna - 1681790 - CRISTIANE MARTINS CARDOSO DE SALLES
Interna - 3145590 - DANIELA COSENTINO GOMES
Externa à Instituição - DANIELA BARROS DE OLIVEIRA - UENF
Externa à Instituição - RAQUEL SOARES CASAES NUNES - UFRA