Banca de DEFESA: NAYANA DE FIGUEIREDO PEREIRA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : NAYANA DE FIGUEIREDO PEREIRA
DATE: 22/07/2022
TIME: 09:00
LOCAL: Google Meet
TITLE:

Evaluation of essential oils of the species
Brazilian Baccharis trimera (Less.) DC) and Mimosa
Benth verrucosa against Ctenocephalides felis felis

KEY WORDS:

Essencial oils. Ectoparasites. Brazilian plants.

PAGES: 75
BIG AREA: Ciências Exatas e da Terra
AREA: Química
SUBÁREA: Química Orgânica
SPECIALTY: Química dos Produtos Naturais
SUMMARY:

Fleas of the species Ctenocephalides felis felis are the most common ectoparasites found in
dogs and cats, which can cause more serious reactions and damage. Studies show that Brazil
presents a growing population of dogs and cats composing family structures, and in case of
manifestations of ectoparasites in pets, this interaction causes transmission to humans. So, the
search for ways to reduce contamination has increased considerably. some oils
essential oils have already been used as an alternative for the control of these ectoparasites, since
which are natural products that cause less damage to the environment. With that, the present
study aims to identify the compounds of essential oils of Brazilian species
Baccharis trimera and Mimosa verrucosa Benth, to evaluate the in vitro activity against the ectoparasite C.
felis felis in all stages of development and to evaluate the toxicity from the eukaryotic model of
Saccharomyces cerevisiae to determine the pulicidal action. Both essential oils showed
terpenic compounds, being the major components found by chromatography analysis
gasa in the essential oil of B. trimera were carquejila acetate (33.0%), β-pinene (5.5%),
β–elemene (5.9%), γ-murulene (5.6) and caryophyllene alcohol (7.0%) and in the essential oil of M.
verrucosa were β-pinene (14.2%), α-pinene (10.7%), sabinene (9.5%), E-caryophyllene
(13.8%) and γ-himachelene (8.1%). Toxicity tests demonstrated a low toxicity to
both oils at a concentration of 0.2 mg/mL, showed no damage to the mitochondria and
demonstrated regular cell growth to the control curve. In vitro assays showed
100% effectiveness against all phases of the life cycle of C. felis felis fleas, being the oil
essential of B. trimera ideal against adult fleas (800 μg.cm-2; LC50= 369.22 μg.cm-2) in 24
and 48h and the essential oil of M. verrucosa ideal against flea larvae (791.2 μg.cm-2; LC50 =
266.29 μg.cm-2 and LC50 = 240.54 μg.cm-2) at 24 and 48h, respectively. It is a study with results
satisfactory and with potential for the development of alternative products from the
bacteria of Baccharis trimera and Mimosa verrucosa in the control against Ctenocephalides felis felis.

BANKING MEMBERS:
Presidente - 1705075 - DOUGLAS SIQUEIRA DE ALMEIDA CHAVES
Interna - 1681790 - CRISTIANE MARTINS CARDOSO DE SALLES
Interno - 1844240 - CRISTIANO JORGE RIGER
Externa à Instituição - VERIDIANA MARIA BRIANEZI DIGNANI DE MOURA
Externa à Instituição - CATHARINA ECCARD FINGOLO