Banca de DEFESA: ROSIANE CONCEIÇÃO DOS SANTOS SIQUEIRA DA SILVEIRA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : ROSIANE CONCEIÇÃO DOS SANTOS SIQUEIRA DA SILVEIRA
DATA : 06/02/2019
HORA: 09:00
LOCAL: PQ - sepropédica
TÍTULO:

CHEMICAL COMPOSITION OF ESSENTIAL OIL OF Schinus molle L. AND ITS ANTITROMBOTIC PROPERTY

PALAVRAS-CHAVES:

Schinus molle, Thrombosis, mirtenol

PÁGINAS: 89
GRANDE ÁREA: Ciências Exatas e da Terra
ÁREA: Química
SUBÁREA: Química Orgânica
ESPECIALIDADE: Química dos Produtos Naturais
RESUMO:

Cardiovascular diseases, especially thrombosis, are associated with ischemic heart disease and stroke, which according to the 2010 Global Disease Burden Study have collectively caused one in four deaths worldwide. Essential oils (OE) have great importance in the development of research, for example synergistic effects with oral anticoagulants have already been reported, indicating a possible antithrombotic property. The objective of this work was to evaluate the chemical composition of Schinus molleL essential oil. and to analyze its activity on hemostasis, as well as monoterpene myrtenol. In order to extract the essential oil from the leaves of Schinus molle L., the hydrodestillation technique was used through the Clevenger apparatus, and the material obtained was characterized by CG-DIC-MS. Anticoagulant action was performed by TTPa and TP tests, and the platelet aggregation assays were performed in an aggregometer following the turbidimetric method of Born & Cross (1963), using ADP and collagen as inductors of aggregation. In the chemical characterization of the OE, 45 substances were identified, the major components being sesquiterpenes (88.77%), of which α-Muurolol and δ-cadinene represented 22.85% and 9.66%, respectively, a result confirmed by the comparison of spectra (5mg.ml-1), using ADP as an inducer, showed higher inhibitory power (80%) and the highest concentration (40mg.ml-1) inhibited 48% of platelet activation. Using collagen as an inducer, OE showed no significant activity, as well as showing activity in the coagulation assays, suggesting that the antithrombotic action of OE is especially targeted via platelet aggregation, possibly by the inhibition of P2 family receptors, which are activated by ADP. Mirtenol showed antiplatelet activity using the two inducers and reached 98.5% inhibition of platelet aggregation, and this was a very satisfactory result, and its inhibition of aggregation was more potent in the induction of collagen. In contrast, mirtenol did not interfere with blood coagulation tests, which directs its antithrombotic activity to the antiplatelet action. The OE of S. molle L. presents mainly sesquiterpenes, besides having antithrombotic activity exclusively through inhibition of platelet aggregation, possibly induced by ADP, because OE does not interfere in the blood coagulation pathways and does not act on the induction by collagen. The monoterpene mirtenol has antithrombotic activity exclusively through the antiplatelet route, being its activity pronounced by the induction of the collagen. These results are very useful for following the studies with other inducers of platelet aggregation and future in vivo studies, in order to promote scientific bases for the development of a herbal medicine that can be used in the prevention or treatment of cardiovascular diseases. This is the first report of the antiplatelet action of Schinus molle L. and the monoterpene myrtenol

MEMBROS DA BANCA:
Presidente - 1705075 - DOUGLAS SIQUEIRA DE ALMEIDA CHAVES
Interno - 1844240 - CRISTIANO JORGE RIGER
Interno - 2582213 - MARCO ANDRE ALVES DE SOUZA
Externo à Instituição - BIANCA FERNANDES GLAUSER - UFRJ
Externo à Instituição - LUCIANA WERMELINGER SERRÃO - UFRJ