Banca de QUALIFICAÇÃO: MARIELE RONDON SANTOS GONÇALVES

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : MARIELE RONDON SANTOS GONÇALVES
DATE: 20/12/2024
TIME: 09:00
LOCAL: UFRRJ-PPGQ (Formato online)
TITLE:

The Art of Separations by Countercurrent Chromatography in Obtaining Bioactive Metabolites from Crateva tapia: Contribution to Brazilian Biodiversity

KEY WORDS:

Crateva tapia, Countercurrent Chromatography, Solvent Selectivity, Antioxidant Activity, Glycation Inhibition Activity.

PAGES: 16
BIG AREA: Ciências Exatas e da Terra
AREA: Química
SUMMARY:

Crateva tapia L., known by names such as cabaceira and trapiá, is a tree belonging to the Capparaceae family, widely distributed across Brazilian biomes such as the Pantanal and Caatinga. With applications ranging from construction to traditional medicine for the treatment of urinary disorders and rheumatism,this species is rich in alkaloids, flavonoids, and saponins, which possessvarious pharmacological properties. The need to distinguish and refine chemicalinformation about Crateva tapia and other species of the genus motivates studies evaluated for chemotaxonomy. This project proposes the use of Countercurrent Chromatography (CCC), a separation technique involving liquid-liquid partitioning without a solid support, to isolate metabolites from the dichloromethane and ethanol extracts of the stem bark, leaves, and roots of C. tapia, with a careful selection of solvent systems (SS) and equipment operational modes (isocratic versus gradient, normal or reverse elution). Complementary techniques, such as CCC-DAD, and, if necessary, LC-MS/MS, will be employed to purify and analyze the isolated compounds, ensuring the required purity level for biological activities. Additionally, analysis using the coupled mass spectrometry system will be complemented by other physical analytical techniques, such as 1H and 13C nuclear magnetic resonance (NMR), both one- and two-dimensional, to identify the constituent substances in the
fractions. This study also aims to explore the antioxidant potential and markers of glycoxidative stress in diabetic complication models.

COMMITTEE MEMBERS:
Externa à Instituição - Karen de Jesus Nicácio - UFMT
Externo à Instituição - Rodrigo Rodrigues de Oliveira - UENF
Presidente - 2181682 - MARCIA CRISTINA CAMPOS DE OLIVEIRA