Banca de DEFESA: ELISABETH ALVES DUARTE PEREIRA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : ELISABETH ALVES DUARTE PEREIRA
DATE: 30/09/2022
TIME: 13:30
LOCAL: PQ ou biblioteca/ UFRRJ (a confirmar)
TITLE:

Prospection of wild sage (Varronia curassavica Jacq.) in Atlantic Forest fragments in the states of Rio de Janeiro and Espírito Santo, in search of genotypes with higher b-caryophyllene and a-humulene content and biological properties

KEY WORDS:

Boraginaceae, Varronia curassavica, chemical diversity, bioactivity, aromatic plants, volatiles.

PAGES: 122
BIG AREA: Ciências Exatas e da Terra
AREA: Química
SUBÁREA: Química Orgânica
SPECIALTY: Química dos Produtos Naturais
SUMMARY:

The wild sage (Varronia curassavica, Boraginaceae) is a native species that has medicinal properties and, therefore, of interest to the pharmaceutical industry. Little is known about the characteristics of the wild sage, especially those related to the essential oil extracted from its leaves. In this context, the chemical diversity of the essential oil of 36 plants of wild sage was studied, collected in coastal regions of Rio de Janeiro and Espírito Santo, in restinga vegetation. The plant material was stabilized by reducing the moisture content to around 15% and then the essential oil was extracted by hydrodistillation and subjected to chromatographic analysis (GC-DIC and GC-MS). After chemical characterization of essential oils, data were organized and subjected to descriptive and multivariate statistical analysis (ACP and AAH). The toxicity of the essential oils extracted against the insect pest Callosobruchus maculatus, the phytopathogenic fungus Pestalotia sp. and the epimastigote form of the protozoan Trypanosoma cruzi. The essential oil was also evaluated for its insecticidal and fungicidal toxic activity, and it was possible to observe a variation in the essential oil content from 0.34 to 4.20% and a total of 177 compounds identified. The highest essential oil contents were observed in EB24 (4.20%) and EB41 (3.93%) plants. The substances with the highest frequencies and highest concentrations were also highlighted, they are α-Humulene, Caryophyllene Oxide, Germacrene D, (2E,6Z)-Farnesol, Shyobunol and 2,3-dihydro-Farnesol. The analysis of hierarchical clusters discriminated the formation of 5 distinct groups: group 1 ((2E, 6Z)-Farnesol), group 2 (Shyobunol and Germacrene D-4-ol), group 3 (α-Santalene), group 4 (β -caryophyllene, Elemol and (2Z, 6E)-Farnesol) and group 5 (β-caryophyllene; 1,10-di-epi-Cubenol and 2,3-dihydro-Farnesol). On the other hand, when applying the criteria of dominance among the majority compounds (proposed by the research group), we observed only four different chemotypes (qt) among the 36 individuals studied: Shyobunol, Germacrene D (30.9; 17.3%); Shyobunol, Cubebol, Germacrene D (23.5; 12.3; 11.7%); Shyobunol, α-Santalene, Germacrene D (15; 14; 11.7%) and Shyobunol (31.7%). The essential oils tested did not show relevant toxic activity against the species C. maculatus and Pestalotia sp., on the other hand, two chemotypes were toxic against the epimastigote form of T. cruzi. These results point to a great intraspecific chemical diversity among the plants studied in situ, indicating that there are genotypes with potential to be explored from an agronomic and biological point of view against T. cruzi.

BANKING MEMBERS:
Presidente - 2582213 - MARCO ANDRE ALVES DE SOUZA
Externo ao Programa - 1809123 - ANDRE MARQUES DOS SANTOS - UFRRJExterno ao Programa - 1712769 - PEDRO CORREA DAMASCENO JUNIOR - UFRRJExterno à Instituição - LEOPOLDO CLEMENTE BARATTO - UFRJ
Externo à Instituição - Rafael Ferreira Da Silva - UFF