Banca de DEFESA: JULIANA PAES LEME DE MELLO SOUZA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
DISCENTE : JULIANA PAES LEME DE MELLO SOUZA
DATA : 08/08/2019
HORA: 13:30
LOCAL: Sala 50- Professor Otto Gotlieb- Instituto de Química
TÍTULO:

Study of the chemical composition of Brazilian brown propolis and the application of extracts of propolis in films of sodium carboxymethylcellulose

PALAVRAS-CHAVES:

brown propolis, phenolic substances, antioxidant capacity hydrogels.

PÁGINAS: 24
GRANDE ÁREA: Ciências Exatas e da Terra
ÁREA: Química
SUBÁREA: Química Orgânica
ESPECIALIDADE: Química dos Produtos Naturais
RESUMO:

Propolis is a natural product that has been used in the treatment of diseases since ancient times. The presence of several phenolic substances explains, in part, the variety of biological and therapeutic properties reported in the literature. Of note are flavonoids, cinnamic acids and their esters. Aiming to expand and produce knowledge about Brazilian propolis, it is necessary to analyze this product in order to determine its identity, its composition and evaluate its effects on health when adopted by the population. The objective of this work was to evaluate the chemical profile, phenolic substances content, antioxidant capacity, comparison of UV-Vis spectral data profiles and antimicrobial activity against staphylococcus aureus of ten samples of  brown propolis (Apis mellifera) obtained from the municipality of Japira north of Paraná. As well as the preparation and characterization of sodium carboxymethylcellulose films impregnated with extracts of brown propolis. The extracts from the propolis samples were prepared using the ethanol solvent P.A (EEP) and methanol P.A (EMP) by four extraction methods: dynamic maceration at room temperature; dynamic maceration at 50ºC; extraction in the ultrasound bath and at room temperature and extraction with ultrasound by immersion probe. It was observed a great variation in the results of the extracts for the yields (% m / m), total phenolics, total flavonoids and antioxidant capacity according to the solvent and the extraction methodology. The extracts of propolis that presented higher contents in phenolics and total flavonoids, do not always present the highest potential antioxidants. The qualitative chemical profile of the propolis extracts were similar and the main substances identified were: chlorogenic, caffeic, ferulic, p-coumaric and rosmarinic, besides flavonoids pinobanksin, canferol, canferide. Although the chemical profile by HPLC-DAD was similar to the chemometric analysis showed difference between the solvents used as well as between types of propolis. Regarding the antimicrobial activity against Staphylococcus aureus, all the propolis extracts tested were effective in promoting inhibition of S. aureus growth in the agar diffusion test. The percent inhibition was similar between the samples. The study of incorporation of propolis with carboxymethylcellulose (NaCMC) was efficient.

MEMBROS DA BANCA:
Externo à Instituição - DAVYSON DE LIMA MOREIRA
Externo à Instituição - MARIA RAQUEL GARCIA VEJA - UENF
Externo à Instituição - ROBERTO CARLOS CAMPOS MARTINS - UFRJ
Presidente - 1177598 - ROSANE NORA CASTRO
Interno - 1700427 - YARA PELUSO CID