Banca de DEFESA: MIRZA NALESSO GOMES SANCHES
Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.DISCENTE : MIRZA NALESSO GOMES SANCHES
DATA : 25/07/2019
HORA: 13:00
LOCAL: Sala 50
TÍTULO:
Phytochemical and Biological Study of Simira grazielae (Rubiaceae)
PALAVRAS-CHAVES:
Simira grazielae, Rubiaceae, Dereplication
PÁGINAS: 285
GRANDE ÁREA: Ciências Exatas e da Terra
ÁREA: Química
SUBÁREA: Química Orgânica
ESPECIALIDADE: Estrutura, Conformação e Estereoquímica
RESUMO:
The study species Simira grazielae P., is popularly known as arariba-red and is distributed in Brazil by the states of BA and ES. The objective of the present study was to contribute to the knowledge of the chemical composition of S. grazielae, Rubiaceae family, through the phytochemical and pharmacological study of extracts, fractions and secondary metabolites isolated in greater quantity. The vegetal material of the wood and bark was collected in the Forest Reserve of Companhia Vale do Rio Doce - ES, later dried at room temperature, crushed and subjected to the exhaustive maceration process with methanol, without heating. The methanolic extracts were subjected to the liquid / liquid partitions with n-hexane, dichloromethane, ethyl acetate and n-butanol, and a small amount of these partitions, together with the extract, were subjected to chemical prospecting. The fractions collected from the partition were chromatographed on classic silica gel exclusion columns (Sephadex LH-20) and analyzed by 1H and 13C NMR, GCMS and LC-MS-IES. In addition to the fractionation, extracts and partitioned fractions were submitted to the analysis by dereplication in the UPLC-ESY-QTOF-MS/MS system in the positive mode, a fast and precise analysis to characterize the chemical profiles. The chemical prospecting tests showed positive results for the metabolites: saponins, steroids, triterpenoids, purines, coumarin derivatives, alkaloids, reducing sugars, saccharides and non-protein amino acids. Eightenn (18) substances were isolated and identified from the wood of S. grazielae: N-acetyl serotonin, Maxonine, Harmana, cis- N-p-coumaroyl Serotonin, trans-N-p-coumaroyl serotonin, N-isoferuloyl serotonin, N-feruloil serotonin (Moschamine), cis-moschamine, N-cafeloyl serotonin, Higarine, Ofiorin B, Ofiorin A, Strytosidic acid, Strictosidene, Desmethylisopauridiantoside Acid unpublished, mixture Triacylglycerides, 5- hydroxindole-3-aldehyde and 6'-O-vanilloylbarbutine, involving sixteen alkaloids of the indole and β-carbolin class. The dereplication technique and the fragmentation proposals allowed postulating the presence of 30 substances in the Dichloromethane fraction, 38 in the ethyl acetate, 14 in the butanolic and 7 in the residual wood and 21 in the bark extract. Some substances appeared in several fractions. The crude extracts and partitions revealed anti-inflammatory and antinociceptive activity, and the acaricide activity investigated with Rhipicephalus microplus larvae did not provide a positive result. The acetate, butanolic and Ofiorin B fractions showed no inhibition or activation effect in the evaluation of the effect on the enzyme tyrosinase. Thus, in this work the identification of 18 substances isolated from the wood of S. grazielae is registered and it is proposed the additional presence of 54 substances through the technique of deregulation involving mainly alkaloids, in addition to revealing the presence of fractions of the species with anti inflammatory and antinociceptive.
MEMBROS DA BANCA:
Presidente - 070.100.018-04 - RAIMUNDO BRAZ FILHO - OUTRO
Interno - 2181682 - MARCIA CRISTINA CAMPOS DE OLIVEIRA
Externo ao Programa - 1184107 - HELENA REGINA PINTO LIMA
Externo ao Programa - 1674073 - BRUNO GUIMARAES MARINHO
Externo à Instituição - Marise Maleck de Oliveira
Externo à Instituição - ROBERTO CARLOS CAMPOS MARTINS - UFRJ
Externo à Instituição - TEREZA AUXILIADORA DO NASCIMENTO RIBEIRO - UFMT