Banca de DEFESA: CAROLINA PASSOS DA CUNHA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
DISCENTE : CAROLINA PASSOS DA CUNHA
DATA : 17/12/2018
HORA: 09:00
LOCAL: Instituto de Química
TÍTULO:

Phytochemical study of Myrcia multiflora (Lam.) DC. E Psychotria
leiocarpa Cham. & Schlitdl.

PALAVRAS-CHAVES:

Psychotria leiocarpa, Myrcia multiflora, Acylated anthocyanins

PÁGINAS: 164
GRANDE ÁREA: Ciências Exatas e da Terra
ÁREA: Química
RESUMO:

Brazil has the greatest bíodíversity in the world, but it needs to value and promote knowledge
of local biodiversity. The species M. multiflora (lam) DC., popularly known as "pedra-ume-caá"
(purple fruit), is a native species that belongs to the Myrtaceae family and occurs in Brazil from
the north to Rio Grande do Sul. The species Psychotria leiocarpa Chamo & Schltdl., popularly
known as "cafeeiro do mato" (blue fruit), is a native species that belongs to the Rubiaceae family
and occurs from Paraíba to Rio Grande do Sul. The characteristic coloration of the fruits (purple
and blue, respectively) of both species draws attention to its potential for use as a natural
colorant. The seasonality in which fruiting occurs has generated difficulty in the phytochemical
characterization of fruits of plant species. In this context, the two species remain without
investígation of the special (secondary) metabolites produced in the fruits. Thus, the aim of this
work was to characterize the fruits of M. multiflora and P. leiocarpa. The quantification of
anthocyanins, carotenoids and phenolic acids present in the fruits of M. multiflora was
performed by HPlC-DAD and the antioxidant capacity was evaluated by free radical capture
methods (DPPH and ABTS). The f1avonols present in fruits of this species were isolated by
accelerated liquid chromatography in reversed-phase column. The methanolic extract of P.
leiocarpa fruits was subjected to partítion in chloroform: water, the aqueous fraction was
subjected to HPLC separation on a reverse phase analytical column coupled to the channel
selector valve and the chloroform fraction was subjected to classic chromatography on a normal
phase column. The isolated substances of both species had their structures determined through
the interpretation ofthe spectral data furnished by UV, 1D and 2D NMR and high-resolution MS
(HRMS) involving comparison with data in the literature. Twenty-one substances were identified
for the first time in the fruits of M. multiflora, among which carotenoids, flavonoids and phenolic
acids were characterized. Nineteen of these substances were quantified. The fruits of M.
multiflora presented the third largest antioxidant capacity when compared to eighteen native
fruits. Eight substances were isolated from the fruits of P. leiocarpa, characterized by
anthocyanins, phenolic acid and steroids, which were identified for the first time in fruits of this
species, three of them characterized as acylated anthocyanins previously unpublished in the
literature. Since the eight substances were identified for the first time in the fruits of this species
and three of these substances are new acylated anthocyanins. The acylated anthocyanins were
identified in P. suterella and P. nuda by comparing the retention time and UV-Vis spectrum with
the extract of P. leiocarpa.

MEMBROS DA BANCA:
Externo à Instituição - HUMBERTO RIBEIRO BIZZO
Interno - 1221906 - LUCIANO RAMOS SUZART
Externo à Instituição - MANUELA CRISTINA PESSANHA DE ARAÚJO SANTIAGO
Interno - 2181682 - MARCIA CRISTINA CAMPOS DE OLIVEIRA
Interno - 070.100.018-04 - RAIMUNDO BRAZ FILHO - OUTRO
Externo à Instituição - RONOEL LUIZ DE OLIVEIRA GODOY