Banca de DEFESA: MARINA WAJSENZON DA FONSECA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : MARINA WAJSENZON DA FONSECA
DATE: 28/01/2021
TIME: 09:30
LOCAL: https://meet.google.com/jku-qrya-cxj
TITLE:

STUDY OF THE ANTIOXIDANT POTENTIAL OF GREEN PROPOLIS, ARTEPELIN C AND ROSMARINIC ACID ON MITOCHONDRIAL FUNCTIONALITY IN Saccharomyces cerevisiae CELLS

KEY WORDS:

Saccharomyces cerevisiae, green propolis, artepillin C, rosmarinic acid, mitochondria

PAGES: 88
BIG AREA: Ciências Biológicas
AREA: Bioquímica
SUBÁREA: Bioquímica dos Microorganismos
SUMMARY:

Propolis is a complex mixture of resinous, gummy and balsamic substances collected by honey bees from any part of the plant tissue, to which bees add salivary secretions, wax and pollen to the final product. Due to the great diversity of Brazilian plant species visited by bees, there is a high variation in the constituent substances of propolis, consequently of their active principles and their coloring. The main vegetable source for the production of propolis from the Southeast region of Brazil is the resin collected from young buds and leaves of Baccharis dracunculifolia L., popularly known as field rosemary. This plant native to Brazil provides high levels of different phenolic substances, such as artepillin C, which is considered a chemical marker for this type of propolis. Flavonoids and phenolic acids are substances derived from the secondary metabolism of plants and correspond to approximately 50% of the constituents of propolis, but this percentage may vary according to the region of product collection. The antioxidant properties of this substance may be associated with phenolic constituents present in its extracts, such as rosmarinic acid and artepelin C. Oxidative stress is related to cardiovascular problems, carcinogenesis and neurodegenerative damage. Reactive oxygen species are generated during cellular respiration leaving mitochondrial DNA vulnerable due to its proximity to the electron transport chain. The mitochondria has some fundamental functions in our organism: cellular respiration, through the electron transport chain; the synthesis of adenosine triphosphate (ATP); the mediation of programmed cell death (apoptosis); and the production of heat; besides having its own DNA (mtDNA). The yeast Saccharomyces cerevisiae is used in experiments as a biological model because it has a cellular organization similar to other eukaryotic cells; its genome fully sequenced; easily grown; low cost. In addition to being optional anaerobics and a model organism for understanding the molecular mechanism of mitochondrial diseases. The objective of this work is to evaluate the antioxidant potential of extracts of green propolis and two of its constituents on mitochondrial activity in S. cerevisiae cells. The results revealed that the treatments, artepillin C, rosmarinic acid and the ethanolic and hexanic extracts, both in the concentration 50 µg.mL-1 , showed a good antioxidant potential, as they were able to decrease lipid peroxidation and increase cell viability in fermentative and non-fermentative medium. The ethanolic extract of green propolis in 50 µg.mL-1 was the only one to contribute positively to the electron transport chain, increasing oxygen consumption during maximum breathing.

BANKING MEMBERS:
Presidente - 1844240 - CRISTIANO JORGE RIGER
Interno - 1700480 - ARTHUR EUGEN KUMMERLE
Interna - 1681790 - CRISTIANE MARTINS CARDOSO DE SALLES
Externa à Instituição - CARMELITA GOMES DA SILVA - IFRJ
Externa à Instituição - ELIS CRISTINA ARAÚJO ELEUTHERIO - UFRJ