Banca de DEFESA: WLADYMIR MENDES CARVALHO DE CASTRO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : WLADYMIR MENDES CARVALHO DE CASTRO
DATE: 02/08/2022
TIME: 09:00
LOCAL: Google Meet
TITLE:

Evaluation of the in vitro activity of the essential oil of Caesalpinia pulcherrima
(L.) Sw. against immature and adult stages of Ctenocephalides felis felis.

KEY WORDS:

essencial oils; pulicides; in vitro tests.

PAGES: 80
BIG AREA: Ciências Exatas e da Terra
AREA: Química
SUBÁREA: Química Orgânica
SPECIALTY: Química dos Produtos Naturais
SUMMARY:

The interaction between humans and animals has increased more and more, and with that, the
concern for the health of these animals. Cats and dogs are susceptible to
infestation by ticks and fleas, with Rhipicephalussanguineus and
Ctenocephalidesfelisfelis the most common. The cat flea, known as C. felisfelis, is

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a hematophagous, wingless insect that parasitizes domestic animals, which need
daily food. In this way, animals constantly suffer from skin irritations,
anemia and allergic dermatitis. Faced with the development of resistance of these insects against
to treatment with chemical products, essential oils are shown as alternatives
promising, since they already have described activity against some insects. Knowing this
potential of essential oils, the present work aims to extract the essential oil
of C. pulcherrima, identify its components, evaluate its insecticidal activity against
adult and immature stages of the cat flea. In vitro screening tests were performed,
to evaluate the behavior of fleas against the essential oil, in 10 concentrations
different in the period of 24 and 48 hours using 10 fleas. The technique was used
impregnation on filter paper. The essential oil of C. pulcherrima is rich in germacrene D
(47.04%) and showed activity against C. felis. Against adults, it was possible to observe
mortality of 15% and 40% at 800 μg cm -2 , within 24 and 48 hours, respectively.
Against eggs and pupae, 100% mortality was observed at the maximum concentration (800 μg cm -
two ); against larvae, maximum mortality was observed at 400 μg cm -2 at both times.
tested. The activity of the essential oil was also tested, in two concentrations, in the inhibition
of the enzyme acetylcholinesterase, but it was not possible to observe this inhibition against the two
analyzed concentrations. The essential oil studied showed significant activity both
for adults and the immature stages of C. felis, concluding that it can be a great
candidate for flea control, being used as an alternative to
development of natural pesticides.

BANKING MEMBERS:
Presidente - 1705075 - DOUGLAS SIQUEIRA DE ALMEIDA CHAVES
Interna - 1681790 - CRISTIANE MARTINS CARDOSO DE SALLES
Externo ao Programa - 1809123 - ANDRE MARQUES DOS SANTOS - UFRRJExterna à Instituição - CATHARINA ECCARD FINGOLO
Externa à Instituição - NEIDE MARA DE MENEZES EPIFANIO