MICROENCAPSULATION OF β-CAROTENE THROUGH COMPLEX COACERVATION USING AS WALL MATERIAL OVALBUMIN AND SODIUM ALGINATE
Bioaccessibility; encapsulated β-carotene; Gastrointestinal simulation; Antioxidant activity.
β-carotene (βC) is a natural fat-soluble compound necessary for human health, being an important food source of provitamin A, but it has high chemical instability, which increases its oxidation in the presence of extrinsic factors. The present study aimed to microencapsulate βC in complex coacervated formed by the interaction between ovalbumin (OVA) and sodium alginate (NaAlg). The microencapsulation technique was used at pH 4.0 and 8:1 ratio (OVA:NaAlg, m/m) after confirming the affinity of these complexes with zeta potential, the study of the phase diagram, turbidimetric and isothermal titration calorimetry analyses. The capsules formed from the OVA:NaAlg complex showed spherical morphology with a well-defined nucleus and high encapsulation efficiency (~97.5 %). The presence of OVA, NaAlg, and βC in the microcapsules was confirmed by Fourier Transform Infrared Spectroscopy (FTIR). The in vitro gastrointestinal digestion simulation of microcapsules found that 71.39 % of the
encapsulated βC was released in the intestine, with a bioaccessibility of 32.78 %. The kinetic profile of release showed, in the first-order model, that the mechanism observed in the release of βC in the food model was diffusion. Antioxidant activity was proven when applying βC microcapsules in the production of cookies, resulting in twice the
protection of the bioactive in relation to free βC. Thus, the results presented suggest that βC microcapsules formed with the OVA:NaAlg wall material can be efficiently inserted in cookies fortification.