Banca de DEFESA: LUIS OTÁVIO MOREIRA SILVA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : LUIS OTÁVIO MOREIRA SILVA
DATA : 21/03/2018
HORA: 09:00
LOCAL: Embrapa Agroindústria de Alimentos
TÍTULO:

DETERMINATION OF THE IN VITRO BIOACESSIBILITY OF THE ROUTINE EXTRACTED BY DIFFERENT PROCESSES OF TOMATO RESIDUE

PALAVRAS-CHAVES:

Key words: tomato; rutin; ultrasound-assisted extraction; conventional extraction; bioaccessibility.

PÁGINAS: 59
GRANDE ÁREA: Ciências Agrárias
ÁREA: Ciência e Tecnologia de Alimentos
RESUMO:

Tomato production in Brazil in 2015 was approximately 4 million tons, which demonstrates its economic relevance. A significant part of the tomato harvest is used as raw material for the production of tomato juice and pulp. However, this industry generates an agroindustry residue that represents 5 to 10% of the used raw material. The tomato residue has been mainly exploited as a source of lycopene. However, this material is also rich in flavonoids such as rutin, which can be applied as an additive in the food industry by promoting health benefits and enhancing this waste. Usually, the extraction process of these compounds is carried out by using traditional techniques using organic solvents, such as hexane, methanol, ethanol, among others, and satisfactory results have been obtained. New methods have been evaluated in order to improve the efficiency of the extraction. Ultrasound-assisted extraction is a method that offers benefits such as ease of operation and higher extraction rate. The objective of this work was to compare two techniques for rutin extraction with regards to the obtained rutin content and the bioaccessibility of this compound. A tomato sample was depulped mimicking the process of obtaining the pulp. The residue, composed of peel and seed, was fractionated and the separated peel was dried at 60°C for 24h, resulting in a powder with a mean size of 483.4±15.7 μm and 1.27±0.23% of moisture content. The powder was characterized for total phenolic content (584.63±1.78 mg gallic acid equivalent/g powdered tomato) and antioxidant capacity by TEAC method (186.98±1.78 μmol Trolox/g tomato powder). The powder was submitted to conventional extraction using 70% (v/v) ethanol at 1:25, 57°C for 40 minutes and ultrasound-assisted extraction when the same extraction solution was used at the same solvent:substrate rate. It was applied for 10 minutes, at 55oC and 150W. The liquid extracts were compared in relation to the antioxidant activity and to the routine content. The antioxidant activity determined by TEAC was higher in the extract obtained by ultrasound (27.90±0.10 μmol Trolox/g). The same behavior was observed for the antioxidant activity, where the extract obtained by ultrasound (109.82 ± 15.03 μmol Trolox / g) showed a slightly higher activity than the conventional extract (97.13±12.44 μmol Trolox/g), but with no statistical difference. The amount of rutine extracted was statistically the same for both methods of extraction (conventional: 3.77±0.014 μg rutin /mL; ultrasound: 3.83±0.065 μg routine / mL). The results demonstrate that the rutin content was not affected by the extraction methods evaluated. Determination of bioavailability is important to identify the percentage of this compound absorbed by the human organism. The bioaccessibility of rutin in both extracts was approximately 13%, suggesting that the extraction method does not influence the absorption of this product. After 4 weeks of storage, the extracts presented a reduction in the accessibility of the rutin. The extracts obtained by the traditional method presented a similar reduction when stored under refrigeration (8.09%) or freezing (8.22%). The reduction in the accessibility of extracts obtained by ultrasound-assisted extraction was lower when stored under the same conditions (refrigeration: 10.1%, freezing: 10.11%). The use of ultrasound-assisted extraction as an alternative for rutin extraction from the residue of tomato processing was not considered technically attractive. 

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