Banca de QUALIFICAÇÃO: MAYARA DOS SANTOS TAVARES

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : MAYARA DOS SANTOS TAVARES
DATE: 08/05/2026
TIME: 09:00
LOCAL: https://meet.google.com/ozt-pshg- jhs
TITLE:

Rhodotorula mucilaginosa: A New Microbial Strain Producing Collagenolytic Protease.

KEY WORDS:

Keywords: collagenase; microorganisms; enzymatic activity; screening.

PAGES: 14
BIG AREA: Ciências Agrárias
AREA: Ciência e Tecnologia de Alimentos
SUBÁREA: Tecnologia de Alimentos
SPECIALTY: Aproveitamento de Subprodutos
SUMMARY:

Proteases are enzymes responsible for protein degradation, including collagenases, which hydrolyze collagen. This study aimed to select a microbial strain with potential for collagenolytic protease production and to optimize pH and temperature conditions for enzyme activity. Twelve strains were evaluated in a medium containing type I collagen as an inducer. Selection was carried out in three stages: plate screening to assess growth and halo formation; Essay I, consisting of submerged fermentation at 30°C for 72 hours, with enzymatic activity determined every 24 hours; and Essay II, conducted under the same conditions, with analyses every 12 hours for the three selected strains. The yeast Rhodotorula mucilaginosa was selected, showing an activity of 0.735 U/L at 24 hours. pH and temperature were optimized using a Central Composite Rotatable Design, with highest activity at pH 4.0 and 50°C, highlighting its potential for biotechnological and industrial applications. 

COMMITTEE MEMBERS:
Presidente - 3333721 - GESILENE MENDONCA DE OLIVEIRA
Externa ao Programa - 1125852 - ELIANE PEREIRA CIPOLATTI - UFRRJExterna à Instituição - ANGELA APARECIDA LEMOS FURTADO