Characterization and Prediction of microbial growth kinetics via stereological practices.
Microbial prediction; Quantitative Stereology; Food safety.
Predictive microbiology uses mathematical models to predict the growth and multiplication of microorganisms and is used as a tool to ensure food safety. The quantitative stereology presents a simple and reliable form of measurements of structures allowing a substitution to the form of determination of the predictive models. The objective of this work was to use stereological measurements as a tool for predictive microbiology, replacing the traditionally used mathematical methods. The objective of this study was to study the fungus Penicillium sp., Which was incubated at 25 ° C and photographed every 2 hours to observe its development. The photos were treated using the ImageJ® program, obtaining the value of the individual area of the fungus (AA), as well as the total area (AT). Thus, through the application of quantitative stereology, it was possible to determine an equation to correlate AA and AT, obtaining the value of the volume fraction. The results allowed the visualization of a new technique for the prediction of microbiological development using the tools of quantitative stereology, making food safety feasible and implementing a new perspective for food preservation analysis and processes.