Banca de DEFESA: RODRIGO FERNANDES CALDEIRA
Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.DISCENTE : RODRIGO FERNANDES CALDEIRA
DATA : 26/07/2018
HORA: 09:30
LOCAL: UNIVERSIDADE FEDERAL RURAL DO RIO DE JANEIRO
TÍTULO:
Effect of maternal supplementation with probiotic (Lactobacillus paracasei) during pregnancy and lactation on the neurobehavioral development Swiss mice offspring
PALAVRAS-CHAVES:
probiotic, Lactobacillus, maternal supplementation, offspring, anxiety
PÁGINAS: 62
GRANDE ÁREA: Ciências Agrárias
ÁREA: Ciência e Tecnologia de Alimentos
RESUMO:
Probiotics are living microorganisms which, when administered in suitable amounts, confer a health benefit on the host. Both nutrition and maternal microbiota can modulate fetal health. However, it is unknown whether probiotics could have a direct effect offspring emotional behavior. Thereby, the objective of our study was to evaluate whether maternal supplementation with acid lactic bacteria is able to alter neurobehavioral parameters of Swiss mice offspring. For this purpose, pregnant mice (n=6 each, and ~35g) were randomly divided into two groups: control and probiotic-treated group. These animals were treated during pregnancy and lactation with milk or milk fermented with Lactobacillus paracasei at a concentration of 107 cells/ml (p.o, daily). After the end of the treatment, part of the progeny with 21 days of life was submitted to euthanasia, and the hippocampus was dissected for RNA analysis. After completing 70 days of life, the rest of the pups were submitted to a battery of behavioral tests composed of the following protocols: open field, light dark box, elevated plus maze and tail suspension tests. According to our results, animals whose mothers were treated with fermented milk had a reduction in the expression of GAD 65 (19%, p = 0.006), GAD 67 (25%, p = 0.003) and α3 subunit GABAA (223%, p = 0.01) in the hippocampus. In the adult phase, the offspring of the treated group had higher time in the central area in the open field test (62%, p = 0.04), lower latency to the light side in the light-dark box test (252% p = 0.01) and lower number of decision-making behaviors in the elevated plus maze test (30%, p = 0.02). No significant results were found in the tail suspension test. These results indicate that maternal supplementation with probiotic modulates offspring hippocampal gabaergic system. Such changes could possibly be associated with the reduction of anxiety-like behavior upon reaching adulthood.
MEMBROS DA BANCA:
Presidente - 359403 - ROSA HELENA LUCHESE
Externo à Instituição - SILVIA REGINA MAGALHÃES COUTO GARCIA - UFRJ
Externo ao Programa - 386960 - WELLINGTON DA SILVA CORTES