Banca de QUALIFICAÇÃO: VANESSA MORAES RAMALHO CASTRO
Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.STUDENT : VANESSA MORAES RAMALHO CASTRO
DATE: 03/09/2020
TIME: 14:30
LOCAL: on line
TITLE:
Microbial interactions of lactobacilli and bifidobacteria strains isolated from the human microbiome, in the probiotic activity related to the control of type 2 diabetes
KEY WORDS:
bacterial interactions; probiotics; bifidobacteria; diabetes mellitus
PAGES: 41
BIG AREA: Ciências Agrárias
AREA: Ciência e Tecnologia de Alimentos
SUBÁREA: Ciência de Alimentos
SPECIALTY: Microbiologia de Alimentos
SUMMARY:
The intestinal microbiota plays an important role in human health and disease. It has been shown that part of this microbiota beneficially affects the metabolism and energy consumption of its hosts, especially in the digestion and absorption of nutrients, participating in a kind of co-metabolism. Certain bacterial populations, such as lactic acid bacteria, have been shown to positively influence health. The upper portion of the gastrointestinal tract (TGI) houses a very low number of microorganisms, with less than 1000 bacterial cells per gram of content, with the predominant microorganisms being lactobacilli and streptococci. However, the colon is densely populated by anaerobes with up to 1012 cells per gram of luminal content. Among the lactic acid bacteria the dominant anaerobic genus is Bifidobacterium, while among facultative anaerobes, lactobacilli from the genus Lactobacillus, Lacticaseibacillus, and Limosilactobacillus. The proportion and number of these bacteria can vary, depending on several genetic and environmental factors, including the state of the disease and food intake. The survival and maintenance of Bifidobacterium is also dependent on interactions with other microorganisms. It was observed that the survival and growth of these bacteria are improved in the presence of lactobacilli or streptococci suggesting a type of mutualistic interaction. The aim of this research is to uncouple bifidobacteria naturally associated with lactobacilli isolated from the human intestinal microbiome. Bifidobacterial strains and associated lactobacilli were isolated from the feces of infants up to 21 days of age by direct plating on MRS agar containing antimicrobials. The associated microorganisms will be decoupled by sonication. The strains of bifidobacteria will be identified genotypically by PCR-RFLP. The axenic growth of each strain and its respective pairs will be evaluated, in vitro and in vivo to identify the most promising microbial interaction. In this work, the effect of the administration of these combinations of microorganisms as well as the strains in axenic culture will be evaluated with in vitro and in vivo tests, using diabetic rats, considering biochemical, inflammatory and hormonal parameters related to the control of diabetes.
BANKING MEMBERS:
Presidente - 359403 - ROSA HELENA LUCHESE
Externo à Instituição - ANDRE FIORAVANTE GUERRA - CEFET/RJ
Externa à Instituição - SILVIA REGINA MAGALHÃES COUTO GARCIA - UFRJ