Antimicrobial and Physicochemical of cassava starch films with microencapsulated Thyme (Thymus vulgaris)
Food Packaging, Active Packaging; Microencapsulation; Complex Coacervation
The present study evaluated the effect of incorporating thyme oil, directly and in microencapsulated form, into cassava starch-based films. Initially, the antimicrobial activity of the oil against a gram-positive (Staphylococcus aureus), a gram-negative (Escherichia. coli) and a fungus (Penicillium sp.) bacteria was performed. The particles were produced by complex coacervation with gum arabic and gelatin and the films were produced by casting technique and characterized regarding physical, chemical and structural properties. The films with thyme oil microparticles showed higher antimicrobial activity than the free oil against the tested microorganisms, and from 5% microparticles on, the films had activity for all microorganisms tested, indicating that the oil encapsulation is efficient to protect its bioactive compounds. Films with 5% and 10% microparticles, as well as films with 5% and 10% free oil showed forming ability, however, a decrease in solubility and an increase in hydrophobic characteristics were observed. However, the oil reduced the tensile strength and elongation, while the microcapsules did not influence the mechanical properties compared to the control film. From the microstructure analysis, changes were observed in the roughness and surface of the film after the addition of oil both directly and in the microencapsulated form, suggesting the possible application of cassava starch-based films incorporated with thyme oil microparticles as biodegradable packaging