Banca de QUALIFICAÇÃO: MAYARA DOS SANTOS TAVARES

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : MAYARA DOS SANTOS TAVARES
DATE: 04/03/2024
TIME: 13:00
LOCAL: https://meet.google.com/amf-gfsx-upt
TITLE:

STRATEGIES FOR PRODUCING COLAGENASES ENZYMES MICROBIAL PROCESS FROM SKIN AND SCALE PRODUCTION OF NILE TILAPIA (Oreochromis niloticus).

KEY WORDS:

skin, scale, production of enzymes, collagenases, Nile Tilapia.

PAGES: 40
BIG AREA: Ciências Agrárias
AREA: Ciência e Tecnologia de Alimentos
SUBÁREA: Tecnologia de Alimentos
SPECIALTY: Aproveitamento de Subprodutos
SUMMARY:

The production of Nile Tilapia in Brazil corresponded to 408.4 thousand tons in 2022, considered the fourth largest producer in the world, being the species that represents 66.1% of national production. However, a problem has arisen with solid waste from processing industries, which are rich in organic and inorganic compounds, generating negative environmental impacts when these wastes are discarded into the environment. The amount of waste that is produced by the processing of tilapia fish in the form of gutted fish presentation or fillets, has generated around 60 to 70% of the body weight, and adds value to the skins and scales, a residue rich in macronutrients such as protein, lipids and ash has become a necessity. An alternative use would be as a substrate for the production of microbial enzymes, such as proteases, lipases, trypsin and collagenases, and their application in the food, cosmetics and pharmaceutical industries. The objectives of this study are to produce, obtain and apply an encapsulated enzymatic extract of microbial collagenases produced from Nile Tilapia skin and scale waste, with potential for catalytic action in the food industry, with emphasis on the fish segment. The skin and scales obtained from the processing of the Nile Tilapia process will be treated and used as a substrate by microbial strains, as for the production of the collagenolytic enzyme extract. Species of bacillus subtilis and filamentous fungi will be tested in the selection stage of collagenase-producing strains (screening), and subjected to fermentative kinetics. With the strain selected, a strategy for the fermentation process will be established using experimental statistical designs (Plackett-Burman and DCCR) to determine the best process conditions and cultivation medium for production of the enzyme extract. Upon obtaining the enzymatic extract, a new statistical design will be used to determine the optimal reaction conditions for collagenase enzymatic activity, and its stability in terms of temperature and pH. Next, the encapsulation study of the collagenolytic enzyme extract will begin using the ionic gelation technique. Once you have the encapsulated enzyme extract, its shelf life under refrigeration and freezing will be evaluated. To date, it has been possible to evaluate skin and scale residues through proximate composition analysis (lipids, protein, ash and moisture). With this project, it is expected to select a microbial strain with industrial potential to produce an enzymatic extract based on collagenases with specificity in fish; determine the best conditions in your production process, with the best reaction, stability and storage conditions for the enzyme extract established; obtain a collagen-based product from the application of this enzymatic extract on substrates of fish origin, mainly aimed at the food industry.

COMMITTEE MEMBERS:
Externa ao Programa - 1125852 - ELIANE PEREIRA CIPOLATTI - UFRRJExterno ao Programa - 3467131 - ROMULO CARDOSO VALADAO - UFRRJExterna à Instituição - ANGELA APARECIDA LEMOS FURTADO