DNA detection, clinical analysis and IgG of Toxoplasma gondii in felines from Rio de Janeiro.
Cats, Hematology, PCR
Toxoplasmosis is a cosmopolitan zoonosis caused by the protozoan Toxoplasma gondii. This parasite can affect several species, with felids being the definitive hosts. In cats, the most affected systems are: respiratory, gastrointestinal and neurological. The main objective is to detect the DNA of T. gondii in felines and to know if there are specific symptoms that signal the infection by T. gondii in the species. Carry out clinical analysis (hematology and biochemistry), serological analysis (IgG detection) and risk factors for T. gondii in the studied cats. The diagnosis of T. gondii will be made from serum and blood samples and data will be registered in the system of 200 cats that will be treated at the Feline Sector at UFRRJ and later sent to the LABVET (Laboratory of Veterinary Clinical Pathology). The Modified Agglutination Test will be used for the investigation of IgG antibodies against T. gondii and PCR for DNA detection. Hematological and biochemical tests (Alanine Aminotransferase, Alkaline Phosphatase, Albumin, Urea, Creatinine, Globulin, Total Proteins) will be computed for correlations. Clinical data and risk factors will also be obtained by consulting the clinical records of the animals evaluated and all data tabulated. The serological and molecular detection of T. gondii will be carried out at the Laboratory of Animal Pathology (SAP) at UFRRJ. So far we have had access to the total information and samples of 10 felines. The data obtained were: hematological, biochemical, clinical signs and risk factors.