Banca de QUALIFICAÇÃO: ANA CAROLINA DE SOUZA CAMPOS

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : ANA CAROLINA DE SOUZA CAMPOS
DATE: 08/05/2023
TIME: 09:00
LOCAL: sala 50 do Instituto de Veterinária
TITLE:

HISTOPATHOLOGICAL, IMMUNOHISTOCHEMICAL AND ULTRASTRUCTURAL ANALYSIS OF HEALTHY OMENTUM IN BITCHES, CATS AND RABBITS

KEY WORDS:

Reconstructive surgery; healing; omentum; mylk spots; tissue repair

PAGES: 94
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Clínica e Cirurgia Animal
SPECIALTY: Clínica Cirúrgica Animal
SUMMARY:

The omentum is a tissue present in all mammals and there is clear evidence of its properties in Veterinary Medicine, such as stimulating neovascularization, tissue reconstitution, lymphatic drainage, filling defects and increasing healing, even in sites with infection. Its application occurs in peritoneal and extra-peritoneal lesions with wide use in surgery, but there is scarce information regarding its microscopic analysis and composition, and there are no studies in veterinary medicine that compare the characteristics of the omentum between domestic species. The study of the components of the omentum in animals is due to the need to understand the true value of this tissue, exploring its basic structure, to observe if there are clusters of immune cells, the known “milky spots”, as reported in the human omentum, and to determine the composition and activity of this immune population. For this, samples of omentum fragment measuring 2cm x 2cm were collected, after ligation of the omental vessels with polyglactin 910 thread or polyglycolic acid (3.0), from 15 cats and 15 bitches during elective ovariosalpingohysterectomy surgery. The samples were cut with metzembaum scissors and, depending on the analysis performed, divided into two, one fixed in 10% buffered formalin for the histopathology and immunohistochemistry technique to be performed at the Immunohistochemistry Laboratory of the Pathological Anatomy Sector of the Federal University Rural do Rio de Janeiro (UFRRJ) in order to detect immune cells (B and T lymphocytes, macrophages and endothelial cells); the other sample was fixed in a karnovizk solution for ultrastructural analysis by means of scanning and transmission electron microscopy performed at the Souto-Padrón e Lins Multi-User Microscopy Unit Laboratory of Microbiology at the Federal University of Rio de Janeiro (UFRJ). Also, healthy omentum samples from 12 rabbits were evaluated by histopathology and immunohistochemistry. These rabbits came from an experimental study that evaluated the application of the omentum in cutaneous wounds with free skin grafts carried out at the Laboratory of Experimental Chemotherapy in Veterinary Parasitology at UFRRJ. Based on histopathological, immunohistochemical and ultrastructural analyzes of the healthy omentum of dogs, cats and rabbits, the objective is to determine its basic composition in order to collaborate in the understanding of its functioning and defense mechanisms in these species. It is believed that this study will bring information about the histopathological, immunohistochemical and ultrastructural characteristics of the omentum in these species to support future studies and clarify its real function in the tissue repair process.

COMMITTEE MEMBERS:
Interno - 3015985 - FELIPE FARIAS PEREIRA DA CAMARA BARROS
Interna - 387386 - MARTA FERNANDA ALBUQUERQUE DA SILVA
Interna - 3247698 - TICIANA DO NASCIMENTO FRANCA
Interna - 2606155 - VIVIAN DE ASSUNCAO NOGUEIRA CARVALHO
Externa ao Programa - 3377607 - APARECIDA ALVES DO NASCIMENTO - UFRRJ