Banca de QUALIFICAÇÃO: LUMARA RAELI LIGEIRO

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
DISCENTE : LUMARA RAELI LIGEIRO
DATA : 21/12/2018
HORA: 09:00
LOCAL: Salão verde
TÍTULO:

Neuronal lesion markers in the cerebrospinal fluid of dogs with spinal cord injury caused by intervertebral disc disease

PALAVRAS-CHAVES:

Cerebrospinal fluid, intervertebral disc disease, spinal cord injury, dog

PÁGINAS: 30
GRANDE ÁREA: Ciências Agrárias
ÁREA: Medicina Veterinária
SUBÁREA: Clínica e Cirurgia Animal
ESPECIALIDADE: Clínica Veterinária
RESUMO:

Spinal cord injury occurs in different species due to trauma or spinal cord diseases and affects the spinal cord's ability to send and receive information to different degrees, generating sensory, motor and autonomic deficiencies. In dogs, spinal cord compression resulting from intervertebral disc disease is the main cause of spinal cord injury and clinical signs vary depending on the location, degree of compression and chronicity of the disease. Although other factors are discussed, the main prognostic factor of dogs with hernia is still absence of nociception that indicates an unfavorable prognosis. Cerebrospinal fluid (CSF) examination is often the determining factor for the diagnosis not only of infectious diseases, but also of inflammatory, demyelinating, neoplastic and degenerative diseases of the nervous system. Aiming to improve the assessment of the extent of spinal cord injury in the acute phase there is a new approach by measuring specific neural proteins within the CSF. The trauma leads to the release of metabolites and proteins from the nervous tissue in CSF and thus allows the study of biomarkers such as S100 beta calcium binding protein, neuron-specific enolase, neurofilament protein, myelin basic protein, glial fibrillar protein, protein chemotactic monocytes 1 and interleukins 6 and 8 as potential markers of spinal cord injury. The general objective of the study is to correlate the concentrations of markers such as NSE, GFAP, S100β, NFL, IL-6 and MCP_1 present in the cerebrospinal fluid with the degree of severity of thoracolumbar spinal compression symptoms caused by protrusion or extrusion of the intervertebral disc in dogs. The present study should be carried out at the Small Animal Veterinary Hospital of the Federal Rural University of Rio de Janeiro and in private veterinary clinics. Twenty dogs from 3 to 10 years old, without distinction of race, sex and up to 25 kg of weight, will be selected, with spinal compression characterized by protrusion or extrusion of the intervertebral disc with acute presentation of up to 10 days. As controls will be used 4 animals from 3 to 10 years of age of any race or sex and weighing up to 25 kg and without any type of neurological disease, but will undergo general anesthesia for surgical castration. The selected dogs will undergo 1) a detailed clinical and neurological examination, divided into 2 groups according to the severity of the spinal cord compression according to the scales of Olby and Frankel; and 2) CSF collection at the time of the CT scan or at the time of the surgical decompression procedure in animals with hernia or castration in the case of controls. The conventional CSF analysis and quantification of IL-6, IL-10 and MCP_1 / CCL2 markers (by the chemiluminescence technique) and NSE, GFAP, S100b and NFH markers (by real-time PCR) will be performed.

MEMBROS DA BANCA:
Interno - 2318733 - DANIEL DE ALMEIDA BALTHAZAR
Interno - 387386 - MARTA FERNANDA ALBUQUERQUE DA SILVA
Externo ao Programa - 387295 - LUIS CARLOS REIS