Banca de DEFESA: ALEX HERINGER REIS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : ALEX HERINGER REIS
DATE: 27/10/2023
TIME: 09:00
LOCAL: Remoto/ online
TITLE:

Research of Toxoplasma gondii in reproductive tissues and serum of wild cats from Furtada Island, Rio de Janeiro.

KEY WORDS:

felines, T. gondii,  MAT, PCR

PAGES: 60
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Patologia Animal
SPECIALTY: Anatomia Patologia Animal
SUMMARY:

Toxoplasma gondii is an obligate intracellular protozoan. Felines are the definitive hosts. The main objective of this study was to research Toxoplasma gondii in reproductive tissues and serum of wild domestic cats (Felis catus), determining its importance in One Health for the microregion studied. The Ethics Committee (CEUA) approved this field study at the Federal University of Rio de Janeiro (UFRRJ) under protocol number 6422250523/2023 (ID 002533). Serum and tissue samples from the reproductive tract of 52 free-ranging domestic cats from Ilha Furtada, Mangaratiba in the State of Rio de Janeiro, Brazil, were used. For histopathological analysis, tissues from testes, uterus, ovaries, placenta and fetuses were previously fixed in 10% formaldehyde. The samples and files with animal data were provided by the Pathological Anatomy Sector (SAP) at UFRRJ. Serological diagnosis for detection of IgG anti-T. gondii was performed using the Modified Agglutination Test (MAT). Reproductive tissues and blood were also analyzed using histopathology and PCR to identify the parasite, carried out in the Pathological Anatomy Sector. Serum samples from 52 cats were investigated using the Modified Agglutination Test (MAT) to detect anti-T. gondii, using the cutoff point of 1:25. Of the 52 samples analyzed, 40.4% were positive (21/52). With this study it was possible to infer that the cats on Ilha Furtada were exposed to T. gondii and therefore, this disease may be present in this wild population. Due to the unique orographic formation, there is a risk of oocysts being carried to the sea due to the descending relief of the island's terrain. This study is a pioneer in “Ilha dos Gatos”. According to the research, none of the samples evaluated were positive for Toxaplasma gondii, when submitted to histopathological diagnosis or PCR. New studies must be carried out to investigate environmental contamination through oocysts eliminated in feces and possible infections in other animal species, including birds and fish, since occasionally this protozoan can also infect cold-blooded animals. Therefore, this study intends to warn that toxoplasmosis can occur in this population of wild felines on Ilha Furtada and further investigations in this population must be carried out.

COMMITTEE MEMBERS:
Presidente - 2932766 - ANDRESSA FERREIRA DA SILVA SPYRIDES
Interno - 1151827 - BRUNO RICARDO SOARES ALBERIGI DA SILVA
Interno - 2318733 - DANIEL DE ALMEIDA BALTHAZAR
Interno - 3701718 - DANIEL GUIMARAES UBIALI
Externo à Instituição - GIDEÃO DA SILVA GALVÃO - UFRB