Banca de QUALIFICAÇÃO: AGATHA FERREIRA XAVIER DE OLIVEIRA

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : AGATHA FERREIRA XAVIER DE OLIVEIRA
DATE: 03/04/2024
TIME: 09:00
LOCAL: https://meet.google.com/jez-czyd-dsx
TITLE:

Hematological alterations and occurrence of Trypanosoma sp. on the effect of altitude in rodents from Parque Nacional do Itatiaia.

KEY WORDS:

Rodentia; haematological analysis; altitudinal gradient; trypasomatids.

PAGES: 62
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Patologia Animal
SUMMARY:

The diversity of rodents is notable across various habitat types, evoking significant interest in research pertaining to adaptive mechanisms and the transmission of transmissible diseases by these mammalian hosts. The present study aimed to compare the hematological parameters of rodents from two different altitude areas of Itatiaia National Park (lowland, ranging from 800 to 1500 m, and highland, from 2000 to 2500 m), as well as to perform a direct investigation through blood smear observation and detection by Polymerase Chain Reaction (PCR) targeting the 18SSU gene of parasites of the genus Trypanosoma. Hematocrit and eosinophil levels were found to be elevated in male rodents from the lowland region (mean respective values of 50.45 ± 9.83; 310.6 ± 299). Notably, all groups from the lowland region exhibited higher leukocyte counts (♂ mean 15597.5 ± 7402.5; ♀ mean 13454.1 ± 9579.3), attributed to increases in neutrophils (♂ mean 4367.3 ± 3026.8; ♀ mean 3463.4 ± 4193.8), lymphocytes (♂ mean 10441.4 ± 4954.3; ♀ mean 9328.5 ± 6158.0), monocytes (♂ mean 156 ± 74; ♀ mean 452.5 ± 319.9), and eosinophils observed solely in males (mean of 310.6 ± 299). Conversely, animals from the highland region demonstrated elevated total plasma protein levels (♂ mean 6.96 ± 0.8; ♀ mean 6.85 ± 0.72) and platelet counts (mean of 208.1 ± 168.3), with the latter being exclusive to males from this region. Molecular analysis for Trypanosoma species detection was conducted solely on blood samples from rodents in the highland region (N=74), demonstrating a rate of 18.92% (N=14) of positive animals that did not show any type of associated hematological alteration. Among these, 20% (N=7) were males and 16.22% (N=6) were females. Trypomastigote forms were identified in blood smear slides of four animals that also tested positive via PCR. All positive animals belonged to the Akodontini Tribe, encompassing the genera Akodon (Meyen, 1833), Brucepattersonius (Hershkovitz, 1998), Castoria (Pardiñas, Geise, Ventura, Lessa, 2016), and Oligoryzomys (Bangs, 1900). These findings underscore varied hematological profiles among Sigmodontinae specimens across altitudinal gradients, along with the prevalence of Trypanosoma sp. infection even at higher elevations. This discovery advocates for further epidemiological investigations aimed at elucidating the role of these rodents in disease propagation within domestic, wild fauna, and human populations.

COMMITTEE MEMBERS:
Presidente - 2572430 - CRISTIANE DIVAN BALDANI
Interna - 3324429 - ANDRESA GUIMARAES
Interno - 2318733 - DANIEL DE ALMEIDA BALTHAZAR
Interno - 3701492 - HUARRISSON AZEVEDO SANTOS
Externa ao Programa - 1159140 - JULIANA MACEDO RAIMUNDO - UFRRJ