Banca de DEFESA: AGATHA FERREIRA XAVIER DE OLIVEIRA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : AGATHA FERREIRA XAVIER DE OLIVEIRA
DATE: 15/04/2024
TIME: 09:00
LOCAL: videoconferência
TITLE:

Assessment of the effects of altitude on hematological values and research on Trypanosoma hemoparasites in wild rodents from Parque Nacional do Itatiaia.

KEY WORDS:

Rodentia; haematological analysis; altitudinal gradient; trypasomatids.

PAGES: 65
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Patologia Animal
SUMMARY:

The diversity of rodents is remarkable in various types of habitats, arousing considerable interest in research related to adaptive mechanisms beyond the spread of transmissible diseases. This study aimed to evaluate the effects of altitude on hematological values and to investigate hemoparasites of the genus Trypanosoma in wild rodents from Parque Nacional do Itatiaia. A total of 114 rodents, males and females, from low and high-altitude regions, between 800 to 1500m and 2000 to 2500m respectively, were included in the study, using baited traps (SISBIO 74498-7). After capture and immediately following euthanasia, blood samples were collected via cardiac puncture (CEUA/IOC-035/2018). Hematological analyses were conducted on rodents belonging to the Cricetidae Family (Subfamily Sigmodontinae) due to the group's greater representativeness and its close evolutionary proximity. A direct investigation through blood smear observation and detection via Polymerase Chain Reaction (PCR) targeting the 18S rRNA gene of Trypanosoma parasites was also conducted in all individuals from the high-altitude region and in five rodents of the genus Rhipidomys {Tschudi, 1845} from the low-altitude region. Regarding comparative hematological analyses, hematocrit and eosinophil values were higher in male rodents from the low-altitude region. Additionally, an increase in total leukocyte count was attributed to an increase in individual counts of neutrophils, lymphocytes, and monocytes in males and females from this same region (low part). Animals from the high part showed higher values only in relation to total plasma protein and platelet count, with the latter data observed only in males. The detection of Trypanosoma sp. in rodents from the high-altitude region (N=74) demonstrated a rate of 18.92% (N=14), with 20% (N=7) in males and 16.22% (N=6) in females belonging to the genera Akodon {Meyen, 1833}, Brucepattersonius {Hershkovitz, 1998}, Castoria {Pardiñas, Geise, Ventura, Lessa, 2016}, and Oligoryzomys {Bangs, 1900}. From the samples of rodents of the genus Rhipidomys {Tschudi, 1845} from the low part tested in PCR, a positivity rate of 20% (N=1) was observed in a single male individual. Trypomastigote forms were identified in blood smear slides from five animals that also tested positive in PCR. The data from this study indicate the existence of varied hematological patterns among sigmodontine rodents along altitude gradients, as well as the presence of Trypanosoma sp. in wild rodents from both regions of Parque Nacional do Itatiaia. These findings encourage further epidemiological studies aimed at elucidating the role played by these rodents in the spread of diseases in domestic, wild fauna, and human health.

COMMITTEE MEMBERS:
Presidente - 2572430 - CRISTIANE DIVAN BALDANI
Interna - 3324429 - ANDRESA GUIMARAES
Interno - 2318733 - DANIEL DE ALMEIDA BALTHAZAR
Interno - 3701492 - HUARRISSON AZEVEDO SANTOS
Externa ao Programa - 1159140 - JULIANA MACEDO RAIMUNDO - UFRRJExterna à Instituição - CARINA FRANCISCATO - UFJF
Externa à Instituição - ALINE MOREIRA DE SOUZA - UFF