Banca de DEFESA: DIEGO GONZALEZ VIVAS

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : DIEGO GONZALEZ VIVAS
DATE: 11/07/2025
TIME: 13:00
LOCAL: Google meet
TITLE:

Clinicopathological and microbiological evaluations of the healing of contaminated skin wounds treated with hydrogel based on Melaleuca alternifolia essential oil and Aloe vera extract

KEY WORDS:

Wound; Aloe vera; Tea tree; Healing; Bactericide

PAGES: 69
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Patologia Animal
SPECIALTY: Anatomia Patologia Animal
SUMMARY:

The optimization of the healing process is due to veterinary medical knowledge in indicating accurate therapeutic approaches, using appropriate drugs in a rational manner; avoiding adverse drug reactions, poisoning and especially bacterial resistance, leading to higher treatment costs and delayed tissue repair. There is an increasing financial and academic investment in preclinical and clinical studies in the lines of research in pharmacology and biomaterials, aiming at the production of new types of dressings, such as hydrogels and calcium alginate. Also following this line of thought, more and more research is being produced using medicinal plants as alternative treatments for wound healing; due to their antiseptic, bactericidal and healing properties. In this category, Aloe vera (Aloe) and Melaleuca altenifolia (Tea tree) stand out. The aim of this study was to perform clinical-pathological and microbiological evaluations of the healing of contaminated skin wounds treated with hydrogel based on Melaleuca alternifolia essential oil and Aloe vera extract. This experiment was approved by the CEUA of Estácio de Sá University, number 001/2023, approved on January 31, 2023. Twenty-four healthy male Wistar rats weighing between 200 and 300 grams were used. With the animals anesthetized, two skin wounds measuring 1.5 cm in diameter were made in the dorsal region, which were subsequently contaminated with multiresistant strains of Staphylococcus aureus and identified in G1 (cranial wound treated only with 0.9% saline solution); G2 (caudal wound treated with collagenase and chloramphenicol ointment) and G3 (wound treated with phytotherapeutic hydrogel). After proper identification, the animals were separated into G1G2 (cranial wound treated with saline solution and caudal wound with ointment) and G1G3 (cranial wound treated with saline solution and caudal wound with phytotherapeutic hydrogel). The wounds were evaluated for their morphological and morphometric appearance at 3, 7 and 14 days postoperatively. After ethical euthanasia of the animals, the fragments of these wounds were subjected to histological analysis, stained with Hematoxylin-Eosin (HE) and Masson's Trichrome to evaluate the type and quantity of inflammatory infiltrate, angiogenesis and fibroplasia. Abdominal organs such as liver, kidneys and spleen were also separated for structural and anatomopathological evaluation. Microscopic evaluation revealed that G3 wounds presented a higher rate of collagen deposition and reepithelialization than G2 wounds. Morphologically, G3 wounds produced less crust than G2 wounds, which, despite maintaining a drier appearance, the crust produced a barrier that protected against the loss of electrolytes, fluids and the entry of extrinsic harmful agents. Regarding the inflammatory process, wounds treated with the commercial ointment presented a lower rate when compared to those treated with the phytotherapeutic hydrogel. In terms of microbiological control, a 25% reduction in the rate of microorganisms was observed in G2 wounds and a 17% reduction in G3 wounds. Thus, the phytotherapeutic hydrogel proposed in this study presented satisfactory results compared to the ointment, as it demonstrated to have a great healing potential, despite its low bactericidal potential; it may be an option to replace the healing and antimicrobial base..

COMMITTEE MEMBERS:
Presidente - 2606155 - VIVIAN DE ASSUNCAO NOGUEIRA CARVALHO
Interna - ***.073.401-** - PAULA VELOZO LEAL - UFBA
Interna - 2572430 - CRISTIANE DIVAN BALDANI
Interno - 2929022 - SAULO ANDRADE CALDAS
Externo à Instituição - TIAGO DA CUNHA PEIXOTO - UFBA
Externa à Instituição - MARIANA CORREIA OLIVEIRA - UES
Externa à Instituição - SAMAY ZILLMANN ROCHA COSTA