Banca de DEFESA: ANDREY JUNIO MOREIRA FERNANDES

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : ANDREY JUNIO MOREIRA FERNANDES
DATE: 25/06/2025
TIME: 09:00
LOCAL: Vídeoconferência
TITLE:

Effect of Chitosan on Skin Wound Healing in Swine: Anatomopathological and Morphometric Aspects

KEY WORDS:

chitosan, wound healing, skin wound

PAGES: 57
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Patologia Animal
SPECIALTY: Anatomia Patologia Animal
SUMMARY:

Wound healing is a physiological process essential for the recovery of skin lesions and plays a crucial role not only in veterinary medicine but also in overall health. The objective of this study was to evaluate the effect of chitosan, a polysaccharide derived from chitin, on skin wound healing in pigs, focusing on histopathological and morphometric aspects. Two forms of chitosan were compared: aerosolized chitosan (QA) and granulated powder (QG), the latter being a conventionally used treatment. The research followed the ISO 10993-6 standards and guidelines, which provide direction for the biocompatibility assessment of materials used in medical devices, ensuring the reliability and applicability of the results. The methodology involved inducing skin wounds in pigs, followed by the application of treatments with either QA or QG. Macroscopic, histopathological, and morphometric evaluations were performed on days 14 and 28 after wound induction, with parameters including collagen deposition, angiogenesis, reepithelialization, and the inflammatory response in the damaged tissues. The macroscopic evaluation focused on measuring the extent of the lesion, the presence of crusts, hemorrhage, necrotic material, and scarring. No macroscopic differences were observed between the wounds evaluated in the two experimental groups. Histopathological evaluation followed criteria established by the ISO standard, focusing on tissue reactivity, granulation tissue formation, and collagen organization. The results showed that both treatments promoted effective healing, with QA showing slightly higher tissue reactivity in the wounds compared to the control group treated with QG. However, no statistically significant differences were found between the groups regarding collagen deposition or new blood vessel formation (angiogenesis), suggesting that while aerosolized chitosan is associated with a more intense response in the early stages of healing, both treatments result in satisfactory tissue regeneration. The morphometric analyses, which included measuring epithelial thickness and quantifying new blood vessels, showed that reepithelialization was similar in both groups, with a slight trend towards thicker epithelium in the wounds treated with QA, but without statistical differences. The results demonstrated the evolution of healing over time for both formulations, highlighting that aerosolized chitosan induced a more pronounced inflammatory response in the first two weeks, which gradually evolved into a more stable healing phase. This study concludes that chitosan, especially in its aerosolized form, is a promising alternative for the treatment of skin wounds in pigs, showing good efficacy in promoting healing and facilitating field application. However, further studies are needed to validate its effectiveness in different clinical models and to explore the advantages of this formulation compared to other available treatments.

COMMITTEE MEMBERS:
Interna - 2572430 - CRISTIANE DIVAN BALDANI
Interna - 3247698 - TICIANA DO NASCIMENTO FRANCA
Interna - 2606155 - VIVIAN DE ASSUNCAO NOGUEIRA CARVALHO
Externa à Instituição - SAMAY ZILLMANN ROCHA COSTA
Externa à Instituição - VALIRIA CERQUEIRA DUARTE - UFPA