Banca de DEFESA: MÁRIO MENDES BONCI

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : MÁRIO MENDES BONCI
DATA : 26/04/2019
HORA: 09:30
LOCAL: SALÃO VERDE
TÍTULO:

In vitro virulence evaluation of clinical and environmental dermatophytes fungi isolates

PALAVRAS-CHAVES:

Dermatophytes, virulence, clinical, environmental 

PÁGINAS: 72
GRANDE ÁREA: Ciências Agrárias
ÁREA: Medicina Veterinária
SUBÁREA: Patologia Animal
RESUMO:

The dermatophytes are a group of fungi that require keratin for growth, keratinizing the skin and attachments of animals and man. Although not obligatory pathogens, Microsporum gypseum, M. canis and Trichophytonspp. are related to infections in animals, causing classic circular lesions on the skin. Although dermatophytosis by geophilic dermatophytes occurs to a lesser extent, M. gypseum is the main species involved and these frames should be considered. Among the factors that cause dermatophytes to be able to infect animals, we highlight enzymes such as DNase, gelatinase, lipase, keratinase, elastase and collagenase, which were evaluated in this study. The habit of domestic animals remain in close contact with the land, in backyards, public roads and parks, justifies the study of their pathogenicity and the differentiation of the enzymatic profile of clinical and environmental isolates of dermatophytes, which may contribute to control strategies and prevention of dermatophytosis. Our research aims at the clinical and environmental isolation of dermatophyte fungi, evaluating the production of DNase, lipase, gelatinase, keratinase, elastase and collagenase, enzymes related to their virulence and the comparison of the production capacity of these enzymes related to the pathogenicity between clinical and environmental strains. The environmental strains were obtained through the technique described by Vanbreuseghem (1952), which uses the equine sterility mixed with the moist soil, using soil samples from different Brazilian sites. The clinical specimens were obtained from animal hairs and crusts sent to the Veterinary Microbiological Diagnostic Service / UFRRJ and sown in Mycosel ® medium. The enzymatic evaluation of the dermatophytes was performed by spectrophotometer (collagenase, elastase and keratinase) absorbance, plaque degradation halo formation (DNase and lipase) and liquefaction in tubes (gelatinase). The project was filed under CEUA nº 1395270617, under the responsibility of professor Francisco de Assis Baroni and had a large portion funded by the laboratory in which the activities were developed, since it has a good part of the materials and equipment required during the search.

MEMBROS DA BANCA:
Presidente - 386849 - FRANCISCO DE ASSIS BARONI
Externo à Instituição - GISELA LARA DA COSTA - FIOCRUZ
Externo ao Programa - 386268 - SERGIO GASPAR DE CAMPOS