Immunohistochemical analysis of Ki-67 expression in melanomas diagnosed in dogs in the Department of Pathology, Federal Rural University of Rio de Janeiro (1999-2020)
oncology, canine, melanoma, immunohistochemistry
In dogs, melanocytic tumors exhibit an extremely diverse biology and aggressive behavior. In order to study the proliferative index in melanocytic tumors diagnosed in dogs in the Department of Pathology of UFRRJ and to compare these indices with the location and histopathological criteria classically associated with aggression, all the cases of melanoma diagnosed in the period between 1999 and 2018, through a careful reading of histories, autopsy reports or biopsies performed or sent to SAP / UFRRJ, from dogs, as well as the reading of histological fragments on slides or the preparation of new slides, if deemed necessary. The prospective study, between 2019 and 2020, will follow the same pattern and evaluation criteria of the retrospective study. Age, sex and race and tumor (location and size), as well as evaluation of lesions in routine staining with hematoxylin-eosin (HE), relative to defined histopathological parameters, will be considered from each animal. cells, junctional activity, nuclear atypia, lymphoplasmacytic infiltrate necrosis, intraepithelial proliferation, ulceration, stroma and intravascular tumor cells. The proliferation index marked by Ki-67 will be assessed by immunohistochemical examination.