Virulence in vitro of elements of the canine oral micobiota
Fungal infections, periodontitis, microbiological competition, yeasts
MENDES, Clara de Almeida. Virulence in vitro of elements of the canine oral micobiota. 2019. 16f. Dissertation (Master Science in Veterinary Medicine, Animal Pathology and Clinical Sciences). Instituto de Veterinária, Departamento de Microbiologia e Imunologia Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, 2019.
Among the infectious diseases, in dogs, periodontopathies are the most recurrent ones, being described mainly by the affection by bacterial agents. It is known that in addition to the bacteria, yeasts are part of the oral microbiota, which can promote primary disorders or establish secondary challenges. Several studies have addressed the bacterial components of the oral cavity of the dog, however, the same is not seen when dealing with yeasts, in spite of having already been described as causing oral disorders. The health of the oral mucosa reflects on the general state of the dog and its affection can present serious consequences, such as feeding difficulty and the possibility of ascending infections evolving to a systemic picture. In addition, with the increasing contact of dogs with humans, bite wounds are increasingly present, and the identification of the oral cavity components of the dog is important for the elucidation of infections of this origin. The manifestation of fungal infections depends on several factors, such as microbiological imbalance and consequent decrease in the competing activity of commensal microorganisms and immunosuppressive factors. The need to elucidate the pathogens involved in disorders of the dog's oral microbiota is increasing. In order to increase knowledge, this study aims to identify and isolate yeasts from the healthy oral cavity and injured oral cavity of the dog and to test the in vitro virulence of these components. To this end, saliva samples from different sites of the oral cavity of the dog will be collected with sterile swabs for further sample processing, isolation and identification of yeasts, which will be submitted to virulence tests. In addition to contributing to animal welfare in veterinary medicine and for the public health, the objective is to isolate and identify yeast components of the oral cavity of dogs, to compare the healthy oral micobiota and injured oral micobiota, to verify and compare the isolates in vitro and help in the choice of clinical treatment and therapeutic.