Banca de QUALIFICAÇÃO: ELIENE PORTO SAD PINA

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
DISCENTE : ELIENE PORTO SAD PINA
DATA : 24/07/2019
HORA: 10:00
LOCAL: IV
TÍTULO:

Molecular characterization of the inflammatory response in asthma on pasture kept horses

PALAVRAS-CHAVES:

Equine asthma; summer pasture-associated recurrent airway obstruction; cytokines Th1 e Th2

PÁGINAS: 90
GRANDE ÁREA: Ciências Agrárias
ÁREA: Medicina Veterinária
RESUMO:

Currently, under the nomenclature of "Equine Asthma", diseases like inflammatory airway disease (IAD), recurrent airway obstruction (RAO) and summer pasture-associated obstructive pulmonary disease (SPARAO) are grouped together, and represents a spectrum of the small airway inflammatory disease in horses, in many ways resembling human asthma. A common allergy-like respiratory disease in horses, with performance limitation, characterized by chronic cough, nasal secretion and respiratory distress. IAD and RAO are currently pulmonary diseases known for affecting horses in Brazil. In this study we describe a clinical picture, previously unheard in Brazil, similar to those disease reported in the Northern Hemisphere known as Summer Pasture-associated obstructive pulmonary disease (SPARAO). Cytological findings obtained from bronchoalveolar lavage, performed during the summer months, in horses older than 16 years from Universidade Federal Rural do Rio de Janeiro, shows a neutrophilic response as well as curshman spirals in the laminae, as seems in SPARAO. Most horses had tracheal mucus accumulation in endoscopy examination, however not all horse demonstrate increased respiration effort at rest or caught and some just had mild signs of respiratory distress. During immunological characterization response, using real-time quantitative polymerase chain reaction (RT-PCR), horses  with equine asthma presented a mixed immune response of TH1 and TH2, due to increased production of IL-1β interleukins, INF-δ, IL-4, IL-5, IL-6 and IL8, but not TNF-α (known acute phase cytokine), thus supporting the cytological diagnosis. The healthy group (mean age of 7.9 years), had no neutrophil accumulation, but some showed increased tracheal mucus. The difference between the groups was significant, with IL-5 gene expression showing to be 100x higher in horse from asthmatic group. Despite not having a severe clinic, some of the examined horses housed at pasture in Rio de Janeiro, showed evidence of obstructive pulmonary disease, implying that horses in tropical climates can also develop pulmonary obstructive diseases associated with summer pasture.

MEMBROS DA BANCA:
Externo à Instituição - BRUNO GONCALVES DE SOUZA - UFRRJ
Externo ao Programa - 386994 - CARLOS HENRIQUE MACHADO
Externo ao Programa - 3701492 - HUARRISSON AZEVEDO SANTOS
Presidente - 2181699 - PAULO DE TARSO LANDGRAF BOTTEON